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Assay Detail

CHEMBL1781819

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human CRTH2 receptor expressed in HEK293-EBNA cells assessed as inhibition of forskolin-stimulated intracellular cAMP production by [125I]-cAMP scintillation proximity assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293-EBNA
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin D2 receptor 2 (CHEMBL5071)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

New indole amide derivatives as potent CRTH2 receptor antagonists.
Zaghdane H, Boyd M, Colucci J, Simard D, Berthelette C, Leblanc Y, Wang Z, Houle R, Lévesque JF, Molinaro C, Hamel M, Stocco R, Sawyer N, Sillaots S, Gervais F, Gallant M.
Bioorg Med Chem Lett (2011) 21 : 3471 -3474
PubMed 21515053 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.29 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1778523 1 8.70
CHEMBL1778387 1 8.52
CHEMBL561132 MK-7246 1.0 1 8.52
CHEMBL1778388 1 8.40
CHEMBL1778524 1 8.40
CHEMBL1778522 1 8.21
CHEMBL1778525 1 8.10
CHEMBL1778506 1 7.92
CHEMBL1778505 1 7.60
CHEMBL1778521 1 7.52
CHEMBL1778508 1 6.85
CHEMBL1778390 1 6.27
CHEMBL1778510 1 6.20
CHEMBL1778507 1 6.09
CHEMBL1778504 1 5.68
CHEMBL1778389 1 5.60
CHEMBL1778509 1 5.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1778523 IC50 = 2.0 nM 8.70
CHEMBL1778387 IC50 = 3.0 nM 8.52
CHEMBL561132 MK-7246 IC50 = 3.0 nM 8.52
CHEMBL1778388 IC50 = 4.0 nM 8.40
CHEMBL1778524 IC50 = 4.0 nM 8.40
CHEMBL1778522 IC50 = 6.2 nM 8.21
CHEMBL1778525 IC50 = 8.0 nM 8.10
CHEMBL1778506 IC50 = 12.0 nM 7.92
CHEMBL1778505 IC50 = 25.0 nM 7.60
CHEMBL1778521 IC50 = 30.0 nM 7.52
CHEMBL1778508 IC50 = 140.0 nM 6.85
CHEMBL1778390 IC50 = 540.0 nM 6.27
CHEMBL1778510 IC50 = 630.0 nM 6.20
CHEMBL1778507 IC50 = 810.0 nM 6.09
CHEMBL1778504 IC50 = 2100.0 nM 5.68
CHEMBL1778389 IC50 = 2500.0 nM 5.60
CHEMBL1778509 IC50 = 3700.0 nM 5.43