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Assay Detail

CHEMBL1785146

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human histamine H3 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin stimulated cAMP accumulation after 6 hrs
19
Total Activities
19
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type SK-N-MC
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H3 receptor (CHEMBL264)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Heterocyclic replacement of the central phenyl core of diamine-based histamine H3 receptor antagonists.
Swanson DM, Shah CR, Lord B, Morton K, Dvorak LK, Mazur C, Apodaca R, Xiao W, Boggs JD, Feinstein M, Wilson SJ, Barbier AJ, Bonaventure P, Lovenberg TW, Carruthers NI.
Eur J Med Chem (2009) 44 : 4413 -4425
PubMed 19577344 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 16 8.94 9.84
pKb 2 - -
pA2 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL129542 JNJ-5207852 1 9.84
CHEMBL1783889 1 9.62
CHEMBL1783910 1 9.46
CHEMBL1783895 1 9.38
CHEMBL194092 1 9.32
CHEMBL1783905 1 9.31
CHEMBL1783890 1 9.26
CHEMBL1783891 1 9.25
CHEMBL1783897 1 9.24
CHEMBL237087 1 9.22
CHEMBL1783893 1 9.09
CHEMBL1783877 1 8.54
CHEMBL1783909 1 8.31
CHEMBL1783915 1 8.16
CHEMBL351231 1 7.89
CHEMBL260374 THIOPERAMIDE 1 7.20
CHEMBL380697 APLYSAMINE 1 -
CHEMBL462605 PITOLISANT 4.0 1 -
CHEMBL97170 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL129542 JNJ-5207852 Kd = 0.1445 nM 9.84
CHEMBL1783889 Kd = 0.2399 nM 9.62
CHEMBL1783910 Kd = 0.3467 nM 9.46
CHEMBL1783895 Kd = 0.4169 nM 9.38
CHEMBL194092 Kd = 0.4786 nM 9.32
CHEMBL1783905 Kd = 0.4898 nM 9.31
CHEMBL1783890 Kd = 0.5495 nM 9.26
CHEMBL1783891 Kd = 0.5623 nM 9.25
CHEMBL1783897 Kd = 0.5754 nM 9.24
CHEMBL237087 Kd = 0.6026 nM 9.22
CHEMBL1783893 Kd = 0.8128 nM 9.09
CHEMBL1783877 Kd = 2.884 nM 8.54
CHEMBL1783909 Kd = 4.898 nM 8.31
CHEMBL1783915 Kd = 6.918 nM 8.16
CHEMBL351231 Kd = 12.88 nM 7.89
CHEMBL260374 THIOPERAMIDE Kd = 63.1 nM 7.20
CHEMBL380697 APLYSAMINE pA2 - -
CHEMBL462605 PITOLISANT pKb = 9.5 -
CHEMBL97170 pKb = 8.64 -