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Assay Detail

CHEMBL1803433

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]ZM241385 from stabilized human adenosine receptor A2a expressed in HEK293 cells followed by receptor capturing on Biocore chips by SPR method
16
Total Activities
8
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Biophysical mapping of the adenosine A2A receptor.
Zhukov A, Andrews SP, Errey JC, Robertson N, Tehan B, Mason JS, Marshall FH, Weir M, Congreve M.
J Med Chem (2011) 54 : 4312 -4323
PubMed 21661720 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 8 7.71 9.00
Kd 8 7.05 9.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL240624 PRELADENANT 3.0 2 9.50
CHEMBL113142 ZM-241385 2 9.30
CHEMBL1800358 2 9.00
CHEMBL1800357 2 7.90
CHEMBL273094 XANTHINE AMINE CONGENER 2 7.70
CHEMBL431770 ISTRADEFYLLINE 4.0 2 6.70
CHEMBL1355736 THEOPHYLLINE 4.0 2 5.50
CHEMBL113 CAFFEINE 4.0 2 5.30

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL240624 PRELADENANT Kd = 0.3162 nM 9.50
CHEMBL113142 ZM-241385 Kd = 0.5012 nM 9.30
CHEMBL1800358 Ki = 1.0 nM 9.00
CHEMBL240624 PRELADENANT Ki = 1.585 nM 8.80
CHEMBL113142 ZM-241385 Ki = 1.995 nM 8.70
CHEMBL1800357 Ki = 12.59 nM 7.90
CHEMBL1800358 Kd = 12.59 nM 7.90
CHEMBL273094 XANTHINE AMINE CONGENER Kd = 19.95 nM 7.70
CHEMBL273094 XANTHINE AMINE CONGENER Ki = 25.12 nM 7.60
CHEMBL431770 ISTRADEFYLLINE Ki = 199.53 nM 6.70
CHEMBL1800357 Kd = 1000.0 nM 6.00
CHEMBL431770 ISTRADEFYLLINE Kd = 1258.93 nM 5.90
CHEMBL1355736 THEOPHYLLINE Kd = 3162.28 nM 5.50
CHEMBL113 CAFFEINE Ki = 5011.87 nM 5.30
CHEMBL113 CAFFEINE Kd = 25118.86 nM 4.60
CHEMBL1355736 THEOPHYLLINE Ki > 10000.0 nM -