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Assay Detail

CHEMBL1804152

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of NFkappa p65 isolated from nuclear extract of human HeLa cells by ELISA
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Transcription factor p65 (CHEMBL5533)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Resorcylic acid lactones with cytotoxic and NF-κB inhibitory activities and their structure-activity relationships.
Ayers S, Graf TN, Adcock AF, Kroll DJ, Matthew S, Carcache de Blanco EJ, Shen Q, Swanson SM, Wani MC, Pearce CJ, Oberlies NH.
J Nat Prod (2011) 74 : 1126 -1131
PubMed 21513293 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.20 7.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL438139 ROCAGLAMIDE 1 7.12
CHEMBL1077979 1 6.60
CHEMBL1801948 1 6.12
CHEMBL1801950 1 4.94
CHEMBL1099228 ZEAENOL 1 -
CHEMBL1801949 1 -
CHEMBL1801951 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL438139 ROCAGLAMIDE IC50 = 75.0 nM 7.12
CHEMBL1077979 IC50 = 250.0 nM 6.60
CHEMBL1801948 IC50 = 750.0 nM 6.12
CHEMBL1801950 IC50 = 11500.0 nM 4.94
CHEMBL1099228 ZEAENOL IC50 > 50000.0 nM -
CHEMBL1801949 IC50 > 50000.0 nM -
CHEMBL1801951 IC50 > 50000.0 nM -