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Assay Detail

CHEMBL1811532

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of B-Raf V600E mutant in human SK-MEL-28 cells assessed as reduction of ERK phosphorylation after 1 hr
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SK-MEL-28
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase B-raf (CHEMBL5145)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development of potent B-RafV600E inhibitors containing an arylsulfonamide headgroup.
Stellwagen JC, Adjabeng GM, Arnone MR, Dickerson SH, Han C, Hornberger KR, King AJ, Mook RA, Petrov KG, Rheault TR, Rominger CM, Rossanese OW, Smitheman KN, Waterson AG, Uehling DE.
Bioorg Med Chem Lett (2011) 21 : 4436 -4440
PubMed 21733693 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 19 7.57 8.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1808255 1 8.40
CHEMBL1808261 1 8.15
CHEMBL1807711 1 8.15
CHEMBL1808259 1 8.10
CHEMBL1808257 1 8.05
CHEMBL1808260 1 8.00
CHEMBL1808256 1 8.00
CHEMBL1808262 1 7.96
CHEMBL1808258 1 7.68
CHEMBL1808264 1 7.64
CHEMBL1808263 1 7.62
CHEMBL1807710 1 7.28
CHEMBL1807708 1 7.00
CHEMBL1807709 1 6.24
CHEMBL1807712 1 5.33
CHEMBL1808254 1 -
CHEMBL1807705 1 -
CHEMBL1807706 1 -
CHEMBL1807707 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1808255 EC50 = 4.0 nM 8.40
CHEMBL1808261 EC50 = 7.0 nM 8.15
CHEMBL1807711 EC50 = 7.0 nM 8.15
CHEMBL1808259 EC50 = 8.0 nM 8.10
CHEMBL1808257 EC50 = 9.0 nM 8.05
CHEMBL1808260 EC50 = 10.0 nM 8.00
CHEMBL1808256 EC50 = 10.0 nM 8.00
CHEMBL1808262 EC50 = 11.0 nM 7.96
CHEMBL1808258 EC50 = 21.0 nM 7.68
CHEMBL1808264 EC50 = 23.0 nM 7.64
CHEMBL1808263 EC50 = 24.0 nM 7.62
CHEMBL1807710 EC50 = 52.0 nM 7.28
CHEMBL1807708 EC50 = 99.0 nM 7.00
CHEMBL1807709 EC50 = 580.0 nM 6.24
CHEMBL1807712 EC50 = 4731.0 nM 5.33
CHEMBL1808254 EC50 > 20000.0 nM -
CHEMBL1807705 EC50 > 2200.0 nM -
CHEMBL1807706 EC50 > 20000.0 nM -
CHEMBL1807707 EC50 > 10000.0 nM -