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Assay Detail

CHEMBL1815452

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity against human ganglionic alpha3beta4alpha5beta2 nAChR in SHSY5Y cells assessed as inhibition of carbamylcholine induced 86Rb+ efflux
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type SH-SY5Y
Confidence 7 — Homologous single protein target
Curated By Autocuration

Publication

Nicotinic acetylcholine receptor efficacy and pharmacological properties of 3-(substituted phenyl)-2β-substituted tropanes.
Carroll FI, Blough BE, Mascarella SW, Navarro HA, Eaton JB, Lukas RJ, Damaj MI.
J Med Chem (2010) 53 : 8345 -8353
PubMed 21058665 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 5.68 6.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1812744 1 6.24
CHEMBL1812747 1 6.14
CHEMBL1812740 1 5.89
CHEMBL1812748 1 5.85
CHEMBL1812741 1 5.77
CHEMBL1812743 1 5.75
CHEMBL1812739 1 5.75
CHEMBL894 BUPROPION 4.0 1 5.75
CHEMBL1812750 1 5.58
CHEMBL1812746 1 5.51
CHEMBL1812749 1 5.50
CHEMBL1812745 1 5.44
CHEMBL1812742 1 4.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1812744 IC50 = 570.0 nM 6.24
CHEMBL1812747 IC50 = 730.0 nM 6.14
CHEMBL1812740 IC50 = 1300.0 nM 5.89
CHEMBL1812748 IC50 = 1400.0 nM 5.85
CHEMBL1812741 IC50 = 1700.0 nM 5.77
CHEMBL1812743 IC50 = 1800.0 nM 5.75
CHEMBL1812739 IC50 = 1800.0 nM 5.75
CHEMBL894 BUPROPION IC50 = 1800.0 nM 5.75
CHEMBL1812750 IC50 = 2600.0 nM 5.58
CHEMBL1812746 IC50 = 3100.0 nM 5.51
CHEMBL1812749 IC50 = 3200.0 nM 5.50
CHEMBL1812745 IC50 = 3600.0 nM 5.44
CHEMBL1812742 IC50 = 20000.0 nM 4.70