Assay Detail
Binding
CHEMBL1819663
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of Escherichia coli DHFR by spectrophotometry
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Escherichia coli |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Design, synthesis, biological evaluation and X-ray crystal structure of novel classical 6,5,6-tricyclic benzo[4,5]thieno[2,3-d]pyrimidines as dual thymidylate synthase and dihydrofolate reductase inhibitors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 5.32 | 6.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1738741 | — | — | 1 | 6.40 |
| CHEMBL1738740 | — | — | 1 | 6.40 |
| CHEMBL389051 | — | — | 1 | 4.96 |
| CHEMBL1818548 | — | — | 1 | 4.82 |
| CHEMBL169896 | OSI-7904 | 2.0 | 1 | 4.70 |
| CHEMBL225180 | — | — | 1 | 4.66 |
| CHEMBL1818549 | — | — | 1 | - |
| CHEMBL225072 | PEMETREXED | 4.0 | 1 | - |
| CHEMBL34259 | METHOTREXATE | 4.0 | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1738741 | — | IC50 | = | 400.0 | nM | 6.40 |
| CHEMBL1738740 | — | IC50 | = | 400.0 | nM | 6.40 |
| CHEMBL389051 | — | IC50 | = | 11000.0 | nM | 4.96 |
| CHEMBL1818548 | — | IC50 | = | 15000.0 | nM | 4.82 |
| CHEMBL169896 | OSI-7904 | IC50 | = | 20000.0 | nM | 4.70 |
| CHEMBL225180 | — | IC50 | = | 22000.0 | nM | 4.66 |
| CHEMBL1818549 | — | IC50 | > | 20000.0 | nM | - |
| CHEMBL225072 | PEMETREXED | IC50 | = | 2300000.0 | nM | - |
| CHEMBL34259 | METHOTREXATE | IC50 | = | 4.4 | nM | - |