Assay Detail
Functional
CHEMBL1820158
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at human TRPA1 channel expressed in HEK293 cells assessed as calcium influx measured at every 1 second for 60 seconds by fluorescence analysis
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Transient receptor potential cation channel subfamily A member 1 (CHEMBL6007) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Design and pharmacological evaluation of PF-4840154, a non-electrophilic reference agonist of the TrpA1 channel.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 11 | 7.09 | 7.64 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1818218 | — | — | 1 | 7.64 |
| CHEMBL1818214 | — | — | 1 | 7.57 |
| CHEMBL1818210 | — | — | 1 | 7.31 |
| CHEMBL1818209 | — | — | 1 | 7.10 |
| CHEMBL1818217 | — | — | 1 | 6.96 |
| CHEMBL233248 | ALLYL ISOTHIOCYANATE | 1.0 | 1 | 5.95 |
| CHEMBL1818216 | — | — | 1 | - |
| CHEMBL1818215 | — | — | 1 | - |
| CHEMBL1818213 | — | — | 1 | - |
| CHEMBL1818212 | — | — | 1 | - |
| CHEMBL1818211 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1818218 | — | EC50 | = | 23.0 | nM | 7.64 |
| CHEMBL1818214 | — | EC50 | = | 27.0 | nM | 7.57 |
| CHEMBL1818210 | — | EC50 | = | 49.0 | nM | 7.31 |
| CHEMBL1818209 | — | EC50 | = | 79.0 | nM | 7.10 |
| CHEMBL1818217 | — | EC50 | = | 109.0 | nM | 6.96 |
| CHEMBL233248 | ALLYL ISOTHIOCYANATE | EC50 | = | 1120.0 | nM | 5.95 |
| CHEMBL1818216 | — | EC50 | - | — | - | |
| CHEMBL1818215 | — | EC50 | - | — | - | |
| CHEMBL1818213 | — | EC50 | - | — | - | |
| CHEMBL1818212 | — | EC50 | - | — | - | |
| CHEMBL1818211 | — | EC50 | - | — | - |