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Assay Detail

CHEMBL1826230

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of iNOS in mouse RAW264.7 cells assessed as anti-inflammatory activity by measuring maximum inhibition of LPS-induced nitric oxide production after 24 hrs
13
Total Activities
12
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type RAW264.7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase, inducible (CHEMBL3464)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Anti-inflammatory endiandric acid analogues from the roots of Beilschmiedia tsangii.
Huang YT, Chang HS, Wang GJ, Cheng MJ, Chen CH, Yang YJ, Chen IS.
J Nat Prod (2011) 74 : 1875 -1880
PubMed 21846089 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 4.44 5.02
Inhibition 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1821987 ENDIANDRAMIDE A 1 5.02
CHEMBL1821990 2 4.79
CHEMBL225304 PIMAGEDINE 2.0 1 4.58
CHEMBL1821989 1 4.40
CHEMBL1821986 TSANGIBEILIN B 1 4.37
CHEMBL1821993 1 4.31
CHEMBL1821985 TSANGIBEILIN A 1 4.30
CHEMBL1821991 BEILSCHMINOL A 1 4.29
CHEMBL1821992 TSANGIN C 1 4.29
CHEMBL1821994 1 4.28
CHEMBL1821988 1 4.24
CHEMBL227744 NITROARGININE 1.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1821987 ENDIANDRAMIDE A IC50 = 9590.0 nM 5.02
CHEMBL1821990 IC50 = 16400.0 nM 4.79
CHEMBL225304 PIMAGEDINE IC50 = 26550.0 nM 4.58
CHEMBL1821989 IC50 = 39560.0 nM 4.40
CHEMBL1821986 TSANGIBEILIN B IC50 = 42300.0 nM 4.37
CHEMBL1821993 IC50 = 48970.0 nM 4.31
CHEMBL1821985 TSANGIBEILIN A IC50 = 49590.0 nM 4.30
CHEMBL1821991 BEILSCHMINOL A IC50 = 51540.0 nM 4.29
CHEMBL1821992 TSANGIN C IC50 = 51420.0 nM 4.29
CHEMBL1821994 IC50 = 52450.0 nM 4.28
CHEMBL1821988 IC50 = 58210.0 nM 4.24
CHEMBL1821990 Inhibition - % -
CHEMBL227744 NITROARGININE IC50 = 152460.0 nM -