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Assay Detail

CHEMBL1912709

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Binding
Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H4 receptor (CHEMBL3759)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Mepyramine-JNJ7777120-hybrid compounds show high affinity to hH(1)R, but low affinity to hH(4)R.
Wagner E, Wittmann HJ, Elz S, Strasser A.
Bioorg Med Chem Lett (2011) 21 : 6274 -6280
PubMed 21944853 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 5.33 7.73

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL129198 JNJ-7777120 1 7.73
CHEMBL129199 1 6.86
CHEMBL382276 1 6.54
CHEMBL1910378 1 5.23
CHEMBL1910382 1 5.17
CHEMBL296419 ASTEMIZOLE 4.0 1 5.10
CHEMBL1910381 1 5.05
CHEMBL1910386 1 4.98
CHEMBL1910385 1 4.85
CHEMBL1910379 1 4.80
CHEMBL1910383 1 4.75
CHEMBL1910380 1 4.65
CHEMBL1910384 1 4.56
CHEMBL657 DIPHENHYDRAMINE 4.0 1 4.37
CHEMBL511 PYRILAMINE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL129198 JNJ-7777120 Ki = 18.62 nM 7.73
CHEMBL129199 Ki = 138.04 nM 6.86
CHEMBL382276 Ki = 288.4 nM 6.54
CHEMBL1910378 Ki = 5888.44 nM 5.23
CHEMBL1910382 Ki = 6760.83 nM 5.17
CHEMBL296419 ASTEMIZOLE Ki = 7943.28 nM 5.10
CHEMBL1910381 Ki = 8912.51 nM 5.05
CHEMBL1910386 Ki = 10471.29 nM 4.98
CHEMBL1910385 Ki = 14125.38 nM 4.85
CHEMBL1910379 Ki = 15848.93 nM 4.80
CHEMBL1910383 Ki = 17782.79 nM 4.75
CHEMBL1910380 Ki = 22387.21 nM 4.65
CHEMBL1910384 Ki = 27542.29 nM 4.56
CHEMBL657 DIPHENHYDRAMINE Ki = 42657.95 nM 4.37
CHEMBL511 PYRILAMINE Ki < 47.0 nM -