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Assay Detail

CHEMBL1913617

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human recombinant M3 receptor expressed in CHO-K1 cells assessed as inhibition of carbamoyl choline-induced calcium currents after 4 hrs by fluorimetry
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M3 (CHEMBL245)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Inhalation by design: novel tertiary amine muscarinic M₃ receptor antagonists with slow off-rate binding kinetics for inhaled once-daily treatment of chronic obstructive pulmonary disease.
Glossop PA, Watson CA, Price DA, Bunnage ME, Middleton DS, Wood A, James K, Roberts D, Strang RS, Yeadon M, Perros-Huguet C, Clarke NP, Trevethick MA, Machin I, Stuart EF, Evans SM, Harrison AC, Fairman DA, Agoram B, Burrows JL, Feeder N, Fulton CK, Dillon BR, Entwistle DA, Spence FJ.
J Med Chem (2011) 54 : 6888 -6904
PubMed 21870878 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 24 9.27 10.31

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1910848 1 10.31
CHEMBL3545181 TIOTROPIUM BROMIDE 4.0 1 10.29
CHEMBL1910852 1 9.80
CHEMBL1910857 1 9.74
CHEMBL1908368 1 9.72
CHEMBL1910856 PF-03635659 2.0 1 9.70
CHEMBL1910851 1 9.68
CHEMBL1910862 1 9.66
CHEMBL1910846 1 9.60
CHEMBL1910865 1 9.59
CHEMBL1910849 1 9.57
CHEMBL1910850 1 9.29
CHEMBL1910860 1 9.25
CHEMBL1910863 1 9.22
CHEMBL1910866 1 9.15
CHEMBL1910858 1 9.14
CHEMBL1910853 1 9.12
CHEMBL1910847 1 8.80
CHEMBL1910861 1 8.72
CHEMBL1910859 1 8.70
CHEMBL1910854 1 8.64
CHEMBL1910845 1 8.62
CHEMBL1910864 1 8.14
CHEMBL1910855 1 7.96

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1910848 Ki = 0.049 nM 10.31
CHEMBL3545181 TIOTROPIUM BROMIDE Ki = 0.051 nM 10.29
CHEMBL1910852 Ki = 0.16 nM 9.80
CHEMBL1910857 Ki = 0.18 nM 9.74
CHEMBL1908368 Ki = 0.19 nM 9.72
CHEMBL1910856 PF-03635659 Ki = 0.2 nM 9.70
CHEMBL1910851 Ki = 0.21 nM 9.68
CHEMBL1910862 Ki = 0.22 nM 9.66
CHEMBL1910846 Ki = 0.25 nM 9.60
CHEMBL1910865 Ki = 0.26 nM 9.59
CHEMBL1910849 Ki = 0.27 nM 9.57
CHEMBL1910850 Ki = 0.51 nM 9.29
CHEMBL1910860 Ki = 0.56 nM 9.25
CHEMBL1910863 Ki = 0.6 nM 9.22
CHEMBL1910866 Ki = 0.71 nM 9.15
CHEMBL1910858 Ki = 0.73 nM 9.14
CHEMBL1910853 Ki = 0.76 nM 9.12
CHEMBL1910847 Ki = 1.6 nM 8.80
CHEMBL1910861 Ki = 1.9 nM 8.72
CHEMBL1910859 Ki = 2.0 nM 8.70
CHEMBL1910854 Ki = 2.3 nM 8.64
CHEMBL1910845 Ki = 2.4 nM 8.62
CHEMBL1910864 Ki = 7.3 nM 8.14
CHEMBL1910855 Ki = 11.0 nM 7.96