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Assay Detail

CHEMBL1918488

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human full-length BTK expressed in Sf9 cells using FAM-Srctide peptide as substrate after 60 mins by TR-FRET Assay
38
Total Activities
38
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase BTK (CHEMBL5251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Imidazo[1,5-a]quinoxalines as irreversible BTK inhibitors for the treatment of rheumatoid arthritis.
Kim KH, Maderna A, Schnute ME, Hegen M, Mohan S, Miyashiro J, Lin L, Li E, Keegan S, Lussier J, Wrocklage C, Nickerson-Nutter CL, Wittwer AJ, Soutter H, Caspers N, Han S, Kurumbail R, Dunussi-Joannopoulos K, Douhan J, Wissner A.
Bioorg Med Chem Lett (2011) 21 : 6258 -6263
PubMed 21958547 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 38 7.97 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1873475 IBRUTINIB 4.0 1 9.30
CHEMBL1916712 1 9.07
CHEMBL1916713 1 9.07
CHEMBL1916715 1 9.05
CHEMBL1916888 1 8.81
CHEMBL1916892 1 8.74
CHEMBL1916882 1 8.72
CHEMBL1916891 1 8.71
CHEMBL1916714 1 8.60
CHEMBL1916881 1 8.53
CHEMBL1916880 1 8.48
CHEMBL1916718 1 8.40
CHEMBL1916897 1 8.40
CHEMBL1916895 1 8.32
CHEMBL1916896 1 8.30
CHEMBL1916894 1 8.27
CHEMBL1916899 1 8.26
CHEMBL1916902 1 8.18
CHEMBL1916893 1 8.17
CHEMBL1916885 1 8.12
CHEMBL1916901 1 8.02
CHEMBL1916898 1 8.02
CHEMBL1916883 1 7.99
CHEMBL1916887 1 7.98
CHEMBL1916889 1 7.91
CHEMBL1916900 1 7.89
CHEMBL1916884 1 7.88
CHEMBL1916879 1 7.78
CHEMBL1916717 1 7.72
CHEMBL1916906 1 7.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1873475 IBRUTINIB IC50 = 0.5 nM 9.30
CHEMBL1916712 IC50 = 0.847 nM 9.07
CHEMBL1916713 IC50 = 0.847 nM 9.07
CHEMBL1916715 IC50 = 0.884 nM 9.05
CHEMBL1916888 IC50 = 1.55 nM 8.81
CHEMBL1916892 IC50 = 1.82 nM 8.74
CHEMBL1916882 IC50 = 1.89 nM 8.72
CHEMBL1916891 IC50 = 1.93 nM 8.71
CHEMBL1916714 IC50 = 2.52 nM 8.60
CHEMBL1916881 IC50 = 2.96 nM 8.53
CHEMBL1916880 IC50 = 3.33 nM 8.48
CHEMBL1916718 IC50 = 3.99 nM 8.40
CHEMBL1916897 IC50 = 3.98 nM 8.40
CHEMBL1916895 IC50 = 4.83 nM 8.32
CHEMBL1916896 IC50 = 5.03 nM 8.30
CHEMBL1916894 IC50 = 5.35 nM 8.27
CHEMBL1916899 IC50 = 5.51 nM 8.26
CHEMBL1916902 IC50 = 6.58 nM 8.18
CHEMBL1916893 IC50 = 6.76 nM 8.17
CHEMBL1916885 IC50 = 7.56 nM 8.12
CHEMBL1916898 IC50 = 9.53 nM 8.02
CHEMBL1916901 IC50 = 9.53 nM 8.02
CHEMBL1916883 IC50 = 10.3 nM 7.99
CHEMBL1916887 IC50 = 10.4 nM 7.98
CHEMBL1916889 IC50 = 12.2 nM 7.91
CHEMBL1916900 IC50 = 13.0 nM 7.89
CHEMBL1916884 IC50 = 13.2 nM 7.88
CHEMBL1916879 IC50 = 16.8 nM 7.78
CHEMBL1916717 IC50 = 19.1 nM 7.72
CHEMBL1916906 IC50 = 40.0 nM 7.40
CHEMBL1916716 IC50 = 45.3 nM 7.34
CHEMBL1916886 IC50 = 60.6 nM 7.22
CHEMBL1916904 IC50 = 72.0 nM 7.14
CHEMBL1916890 IC50 = 76.5 nM 7.12
CHEMBL1916903 IC50 = 283.0 nM 6.55
CHEMBL1916907 IC50 = 670.0 nM 6.17
CHEMBL1916905 IC50 = 1220.0 nM 5.91
CHEMBL1916719 IC50 = 4290.0 nM 5.37