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Assay Detail

CHEMBL1918596

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Functional
Agonist activity at GPR35 in human HT-29 cells assessed as desensitization of 1 uM zaprinast-induced DMR after 1 hr by dynamic mass redistribution assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

G-protein coupled receptor 35 (CHEMBL1293267)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 2-(4-methylfuran-2(5H)-ylidene)malononitrile and thieno[3,2-b]thiophene-2-carboxylic acid derivatives as G protein-coupled receptor 35 (GPR35) agonists.
Deng H, Hu H, He M, Hu J, Niu W, Ferrie AM, Fang Y.
J Med Chem (2011) 54 : 7385 -7396
PubMed 21950657 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.46 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1914576 1 7.22
CHEMBL1914588 1 6.96
CHEMBL1914590 1 6.24
CHEMBL1914589 1 5.98
CHEMBL28079 ZAPRINAST 2.0 1 5.91
CHEMBL1914585 1 5.83
CHEMBL1914591 1 5.73
CHEMBL1914578 1 5.71
CHEMBL1914577 1 5.35
CHEMBL1914586 1 5.33
CHEMBL1914592 1 5.23
CHEMBL1914587 1 5.22
CHEMBL1914581 1 5.19
CHEMBL1914580 1 4.99
CHEMBL1914584 1 4.92
CHEMBL1914582 1 4.79
CHEMBL1914579 1 4.67
CHEMBL1914583 1 4.31
CHEMBL1173787 1 4.24

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1914576 IC50 = 60.0 nM 7.22
CHEMBL1914588 IC50 = 110.0 nM 6.96
CHEMBL1914590 IC50 = 570.0 nM 6.24
CHEMBL1914589 IC50 = 1050.0 nM 5.98
CHEMBL28079 ZAPRINAST IC50 = 1220.0 nM 5.91
CHEMBL1914585 IC50 = 1490.0 nM 5.83
CHEMBL1914591 IC50 = 1870.0 nM 5.73
CHEMBL1914578 IC50 = 1940.0 nM 5.71
CHEMBL1914577 IC50 = 4480.0 nM 5.35
CHEMBL1914586 IC50 = 4690.0 nM 5.33
CHEMBL1914592 IC50 = 5920.0 nM 5.23
CHEMBL1914587 IC50 = 6070.0 nM 5.22
CHEMBL1914581 IC50 = 6400.0 nM 5.19
CHEMBL1914580 IC50 = 10300.0 nM 4.99
CHEMBL1914584 IC50 = 12100.0 nM 4.92
CHEMBL1914582 IC50 = 16400.0 nM 4.79
CHEMBL1914579 IC50 = 21600.0 nM 4.67
CHEMBL1914583 IC50 = 48900.0 nM 4.31
CHEMBL1173787 IC50 = 57000.0 nM 4.24