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Assay Detail

CHEMBL1920261

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against mouse NIH/3T3 cells transfected with PO4 mutant harboring four Ser to Arg mutation selected at 2400 ug/ml vincristine after 72 hrs by MTT assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type NIH3T3
Confidence 1 — Organism
Curated By Autocuration

Target

NIH3T3 (CHEMBL614822)
Type CELL-LINE
Organism Mus musculus

Publication

Synthesis and structure-activity evaluation of isatin-β-thiosemicarbazones with improved selective activity toward multidrug-resistant cells expressing P-glycoprotein.
Hall MD, Brimacombe KR, Varonka MS, Pluchino KM, Monda JK, Li J, Walsh MJ, Boxer MB, Warren TH, Fales HM, Gottesman MM.
J Med Chem (2011) 54 : 5878 -5889
PubMed 21721528 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 5.61 5.65

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL504574 1 5.65
CHEMBL53463 DOXORUBICIN 4.0 1 5.57

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL504574 IC50 = 2230.0 nM 5.65
CHEMBL53463 DOXORUBICIN IC50 = 2700.0 nM 5.57