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Assay Detail

CHEMBL1926252

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant JAK2 using biotinyl-amino-hexanoyl-EQEDEPEGDYFEWLE-amide as substrate after 20 mins by fluorescence assay
31
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK2 (CHEMBL2971)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

2,7-Pyrrolo[2,1-f][1,2,4]triazines as JAK2 inhibitors: modification of target structure to minimize reactive metabolite formation.
Weinberg LR, Albom MS, Angeles TS, Breslin HJ, Gingrich DE, Huang Z, Lisko JG, Mason JL, Milkiewicz KL, Thieu TV, Underiner TL, Wells GJ, Wells-Knecht KJ, Dorsey BD.
Bioorg Med Chem Lett (2011) 21 : 7325 -7330
PubMed 22041060 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 9.05 9.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1822515 2 9.77
CHEMBL1923571 1 9.77
CHEMBL1923573 1 9.64
CHEMBL1923588 1 9.52
CHEMBL1923585 1 9.48
CHEMBL1923575 1 9.46
CHEMBL1923583 1 9.46
CHEMBL1923580 1 9.44
CHEMBL1923589 1 9.40
CHEMBL1923590 1 9.40
CHEMBL1923574 1 9.33
CHEMBL1923579 1 9.30
CHEMBL1923570 1 9.30
CHEMBL1923584 1 9.30
CHEMBL1923594 1 9.30
CHEMBL1923577 1 9.27
CHEMBL1923593 1 9.22
CHEMBL1923576 1 9.10
CHEMBL1923592 1 9.05
CHEMBL1923586 1 9.00
CHEMBL1923578 1 9.00
CHEMBL1923582 1 8.96
CHEMBL1923569 1 8.94
CHEMBL1923587 1 8.92
CHEMBL1921819 1 8.82
CHEMBL1821735 1 8.80
CHEMBL1923572 1 8.68
CHEMBL1923581 1 8.43
CHEMBL1822525 1 6.42
CHEMBL1923591 1 6.32

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1923571 IC50 = 0.17 nM 9.77
CHEMBL1822515 IC50 = 0.17 nM 9.77
CHEMBL1822515 IC50 = 0.19 nM 9.72
CHEMBL1923573 IC50 = 0.23 nM 9.64
CHEMBL1923588 IC50 = 0.3 nM 9.52
CHEMBL1923585 IC50 = 0.33 nM 9.48
CHEMBL1923583 IC50 = 0.35 nM 9.46
CHEMBL1923575 IC50 = 0.35 nM 9.46
CHEMBL1923580 IC50 = 0.36 nM 9.44
CHEMBL1923589 IC50 = 0.4 nM 9.40
CHEMBL1923590 IC50 = 0.4 nM 9.40
CHEMBL1923574 IC50 = 0.47 nM 9.33
CHEMBL1923594 IC50 = 0.5 nM 9.30
CHEMBL1923570 IC50 = 0.5 nM 9.30
CHEMBL1923579 IC50 = 0.5 nM 9.30
CHEMBL1923584 IC50 = 0.5 nM 9.30
CHEMBL1923577 IC50 = 0.54 nM 9.27
CHEMBL1923593 IC50 = 0.6 nM 9.22
CHEMBL1923576 IC50 = 0.8 nM 9.10
CHEMBL1923592 IC50 = 0.9 nM 9.05
CHEMBL1923586 IC50 = 1.0 nM 9.00
CHEMBL1923578 IC50 = 1.0 nM 9.00
CHEMBL1923582 IC50 = 1.1 nM 8.96
CHEMBL1923569 IC50 = 1.14 nM 8.94
CHEMBL1923587 IC50 = 1.2 nM 8.92
CHEMBL1921819 IC50 = 1.5 nM 8.82
CHEMBL1821735 IC50 = 1.6 nM 8.80
CHEMBL1923572 IC50 = 2.1 nM 8.68
CHEMBL1923581 IC50 = 3.7 nM 8.43
CHEMBL1822525 IC50 = 379.0 nM 6.42
CHEMBL1923591 IC50 = 480.0 nM 6.32