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Assay Detail

CHEMBL1929764

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human MR transfected in human COS1 cells after 1 day by luciferase reporter gene assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type COS-1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mineralocorticoid receptor (CHEMBL1994)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of benzoxazin-3-one derivatives as novel, potent, and selective nonsteroidal mineralocorticoid receptor antagonists.
Hasui T, Matsunaga N, Ora T, Ohyabu N, Nishigaki N, Imura Y, Igata Y, Matsui H, Motoyaji T, Tanaka T, Habuka N, Sogabe S, Ono M, Siedem CS, Tang TP, Gauthier C, De Meese LA, Boyd SA, Fukumoto S.
J Med Chem (2011) 54 : 8616 -8631
PubMed 22074142 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 7.05 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1929042 1 7.82
CHEMBL1929039 1 7.37
CHEMBL1393 SPIRONOLACTONE 4.0 1 7.22
CHEMBL1929041 1 7.16
CHEMBL1929040 1 7.07
CHEMBL1929036 1 6.82
CHEMBL1095097 EPLERENONE 4.0 1 5.89

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1929042 IC50 = 15.0 nM 7.82
CHEMBL1929039 IC50 = 43.0 nM 7.37
CHEMBL1393 SPIRONOLACTONE IC50 = 60.0 nM 7.22
CHEMBL1929041 IC50 = 70.0 nM 7.16
CHEMBL1929040 IC50 = 85.0 nM 7.07
CHEMBL1929036 IC50 = 150.0 nM 6.82
CHEMBL1095097 EPLERENONE IC50 = 1300.0 nM 5.89