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Compound Detail

CHEMBL1393

SPIRONOLACTONE
💊 Approved Drug
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💊 Approved Drug
CC(=O)S[C@@H]1CC2=CC(=O)CC[C@]2(C)[C@H]2CC[C@@]3(C)[C@@H](CC[C@@]34CCC(=O)O4)[C@H]12

Basic Information

ChEMBL ID CHEMBL1393
Name SPIRONOLACTONE
Phase Approved
Structure Type MOL
InChI Key LXMSZDCAJNLERA-ZHYRCANASA-N
Therapeutic ✓ Yes

Known Names

Abbolactone Aldactone Carospir Diatensec Espironolactona Gx spironol Laractone NSC-150399 Qaialdo SC-9420 Spiretic Spiroctan 100 +10 more
13
Targets
50
Activities
3
Assay Types
2
PK Parameters
20
Publications
22
Known Names
20
Indications
1
Mechanisms

Molecular Properties

416.6
MW (Da)
4.85
ALogP
5
HBA
0
HBD
60.4
PSA (Ų)
0.57
QED
0
Ro5 Violations
1
Rot. Bonds

Drug Information

Molecule Type Small molecule
First Approval 1960
Availability Prescription
Chirality Single Enantiomer

Routes of Administration

Oral

Flags

Black Box Warning Natural Product

Extended Properties

Molecular Formula C24H32O4S
MW 416.58
Aromatic Rings 0
Heavy Atoms 29
NP-Likeness 2.25

Mechanism of Action

Mechanism Action Type Target Direct Efficacy
Mineralocorticoid receptor antagonist ANTAGONIST Mineralocorticoid receptor

Indications

Ascites Cardiovascular Diseases Edema Essential Hypertension Fibrosis Heart Failure Heart Failure Hyperaldosteronism Hypertension Liver Cirrhosis Myocardial Infarction Nephrotic Syndrome Toxemia Acne Vulgaris Acute Kidney Injury Aortic Valve Stenosis Arthritis, Rheumatoid Atrial Fibrillation Heart Failure, Diastolic Muscular Dystrophy, Duchenne

ATC Classification

C03DA01
spironolactone
Level 1: CARDIOVASCULAR SYSTEM
Level 2: DIURETICS

Drug Warnings

Black Box Warning
carcinogenicity
Country: United States

Activity Summary

IC50 41 meas. best 8.8
Ki 8 meas. best 8.63
EC50 1 meas. best 5.48

Target Activity Profile

Target Type Best pChEMBL Mean pChEMBL Best (nM) # Data
Mineralocorticoid receptor
CHEMBL1994
IC50 8.8 7.7023 1.6 13
Mineralocorticoid receptor
CHEMBL1994
Ki 8.63 8.465 2.3 2
Glucocorticoid receptor
CHEMBL2034
Ki 7.49 6.775 32.6 2
Androgen receptor
CHEMBL1871
Ki 7.41 7.41 39.4 1
Androgen receptor
CHEMBL1871
IC50 7.32 6.63 48.0 6
Androgen receptor
CHEMBL3072
Ki 7.04 7.04 90.6 1
Androgen receptor
CHEMBL3072
IC50 6.87 6.52 135.9 2
Progesterone receptor
CHEMBL1909044
Ki 6.66 6.66 217.2 1
Progesterone receptor
CHEMBL208
Ki 6.4 6.4 399.7 1
Progesterone receptor
CHEMBL208
IC50 6.19 5.9075 650.0 4
Unchecked
CHEMBL612545
IC50 6.01 6.01 977.0 1
Glucocorticoid receptor
CHEMBL2034
IC50 5.85 5.468 1400.0 5
Progesterone receptor
CHEMBL1909044
IC50 5.78 5.78 1665.4 1
Cytochrome P450 2C19
CHEMBL3622
IC50 5.52 5.52 3000.0 1
Estrogen receptor beta
CHEMBL242
EC50 5.48 5.48 3300.0 1
Estrogen receptor
CHEMBL206
IC50 5.24 5.24 5702.0 1
Plasmodium falciparum
CHEMBL364
IC50 5.0 4.96 10000.0 5
Multidrug and toxin extrusion protein 1
CHEMBL1743126
IC50 4.73 4.73 18600.0 1
ATP-dependent translocase ABCB1
CHEMBL4302
IC50 4.63 4.63 23600.0 1

Pharmacokinetic Data

Parameter Value Units Species
F 49.0 % Homo sapiens
F 25.0 % Homo sapiens

Activity Data Samples

Top measurements by pChEMBL value

Target Type Rel. Value Units pChEMBL Assay PubMed
Mineralocorticoid receptor IC50 = 1.6 nM 8.8 Antagonist activity at mineralocorticoid receptor ligand binding domain expresse... 19863083
Mineralocorticoid receptor Ki = 2.323 nM 8.63 Binding affinity to human mineralocorticoid receptor 18038968
Mineralocorticoid receptor Ki = 5.012 nM 8.3 Displacement of [3H]-aldosterone to human mineralocorticoid receptor LBD by radi... 30596500
Mineralocorticoid receptor IC50 = 8.7 nM 8.06 Antagonist activity at human wild-type mineralocorticoid receptor expressed in C... 35652647
Mineralocorticoid receptor IC50 = 9.62 nM 8.02 Displacement of [3H]-aldosterone from recombinant human mineralocorticoid recept... 35652647
Mineralocorticoid receptor IC50 = 11.0 nM 7.96 Antagonist activity at human mineralocorticoid receptor assessed as inhibition o... 23777778
Mineralocorticoid receptor IC50 = 11.0 nM 7.96 Antagonist activity at mineralocorticoid receptor (unknown origin) by PathHunter... 24630411
Mineralocorticoid receptor IC50 = 11.0 nM 7.96 Antagonist activity at human full-length MCR assessed as inhibition of receptor ... 27161805
Mineralocorticoid receptor IC50 = 13.0 nM 7.89 Antagonist activity at Gal4-tagged mineralocorticoid receptor expressed in human... 20672822
Glucocorticoid receptor Ki = 32.6 nM 7.49 Binding affinity to human glucocorticoid receptor 18038968
Androgen receptor Ki = 39.4 nM 7.41 Binding affinity to human androgen receptor 18038968
Androgen receptor IC50 = 48.0 nM 7.32 Displacement of [3H]-methyltrienolene from human androgen receptor incubated ove... 35652647
Mineralocorticoid receptor IC50 = 49.0 nM 7.31 Displacement of [3H]aldosterone from human mineralocorticoid receptor expressed ... 25187277
Mineralocorticoid receptor IC50 = 49.0 nM 7.31 Displacement of [3H]aldosterone from cytosolic human MR expressed in HEK293 cell... 22074142
Mineralocorticoid receptor IC50 = 50.12 nM 7.3 Antagonist activity at gal4-fused human mineralocorticoid receptor LBD expressed... 30596500
Mineralocorticoid receptor IC50 = 60.0 nM 7.22 Antagonist activity at human mineralocorticoid receptor expressed in COS1 cells ... 25187277
Mineralocorticoid receptor IC50 = 60.0 nM 7.22 Antagonist activity at human MR transfected in human COS1 cells after 1 day by l... 22074142
Mineralocorticoid receptor IC50 = 76.0 nM 7.12 Antagonist activity at mineralocorticoid receptor (unknown origin) transfected i... 36154033
Androgen receptor Ki = 90.6 nM 7.04 DRUGMATRIX: Androgen (Testosterone) AR radioligand binding (ligand: [3H] Miboler... -
Androgen receptor IC50 = 120.0 nM 6.92 Displacement of [3H]testosterone from androgen receptor (unknown origin) express... 25187277

Literature References

PubMed DOI Target Context # Activities
- 10.6019/CHEMBL3885881 Rattus norvegicus 332
3612688 10.1021/jm00391a023 Androgen receptor 23
16472241 10.2174/1570163043334794 Hepatotoxicity 18
22194678 10.1371/journal.pcbi.1002310 Hepatotoxicity 14
15646539 10.1016/s0399-8320(04)95062-2 Unchecked 11
2299621 10.1021/jm00164a007 Androgen receptor 9
22861813 10.1021/jm300663n Mus musculus 9
15646539 10.1016/s0399-8320(04)95062-2 NON-PROTEIN TARGET 8
3612688 10.1021/jm00391a023 Progesterone receptor 7
3040999 10.1021/jm00392a022 Mineralocorticoid receptor 7
2984418 10.1021/jm00382a007 Mineralocorticoid receptor 5
19734910 10.1038/nchembio.215 Plasmodium falciparum 5
2984418 10.1021/jm00382a007 Androgen receptor 4
37468498 10.1038/s41467-023-40064-9 Alpha-1A adrenergic receptor 4
20014752 10.1021/tx900326k Hepatotoxicity 3
3989815 10.1021/jm50001a002 Progesterone receptor 3
25187277 10.1016/j.bmc.2014.07.038 Rattus norvegicus 3
25187277 10.1016/j.bmc.2014.07.038 Mineralocorticoid receptor 3
2299621 10.1021/jm00164a007 Progesterone receptor 3
3040999 10.1021/jm00392a022 Androgen receptor 3

Data from ChEMBL 36 via Core Engine