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Assay Detail

CHEMBL4717253

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human full-length MCR assessed as inhibition of receptor binding to co-activator peptide by PathHunter assay
30
Total Activities
30
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mineralocorticoid receptor (CHEMBL1994)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of novel non-steroidal reverse indole mineralocorticoid receptor antagonists.
Ogawa AK,Bunte EV,Mal R,Lan P,Sun Z,Crespo A,Wiltsie J,Clemas J,Gibson J,Contino L,Lisnock J,Zhou G,Garcia-Calvo M,Jochnowitz N,Ma X,Pan Y,Brown P,Zamlynny B,Bateman T,Leung D,Xu L,Tong X,Liu K,Crook M,Sinclair P
Bioorg Med Chem Lett (2016) 26 : 2866 -2869
PubMed 27161805 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 30 6.38 7.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1393 SPIRONOLACTONE 4.0 1 7.96
CHEMBL4792628 1 7.82
CHEMBL4439903 1 7.68
CHEMBL4759836 1 7.52
CHEMBL4798697 1 7.48
CHEMBL4764165 1 7.48
CHEMBL4794194 1 7.22
CHEMBL4777170 1 7.16
CHEMBL4776613 1 7.01
CHEMBL4563704 1 6.94
CHEMBL4790021 1 6.70
CHEMBL4746794 1 6.70
CHEMBL1095097 EPLERENONE 4.0 1 6.61
CHEMBL4755269 1 6.56
CHEMBL4749536 1 6.48
CHEMBL4742925 1 6.28
CHEMBL4754975 1 6.03
CHEMBL4796191 1 6.02
CHEMBL4748904 1 5.77
CHEMBL4792176 1 5.72
CHEMBL4747722 1 5.72
CHEMBL4760159 1 5.72
CHEMBL4759690 1 5.67
CHEMBL4756852 1 5.64
CHEMBL4777887 1 5.57
CHEMBL4746832 1 5.40
CHEMBL4798873 1 5.32
CHEMBL4763926 1 5.10
CHEMBL4794041 1 5.01
CHEMBL4780739 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1393 SPIRONOLACTONE IC50 = 11.0 nM 7.96
CHEMBL4792628 IC50 = 15.0 nM 7.82
CHEMBL4439903 IC50 = 21.0 nM 7.68
CHEMBL4759836 IC50 = 30.0 nM 7.52
CHEMBL4798697 IC50 = 33.0 nM 7.48
CHEMBL4764165 IC50 = 33.0 nM 7.48
CHEMBL4794194 IC50 = 60.0 nM 7.22
CHEMBL4777170 IC50 = 70.0 nM 7.16
CHEMBL4776613 IC50 = 97.0 nM 7.01
CHEMBL4563704 IC50 = 115.0 nM 6.94
CHEMBL4790021 IC50 = 200.0 nM 6.70
CHEMBL4746794 IC50 = 200.0 nM 6.70
CHEMBL1095097 EPLERENONE IC50 = 244.0 nM 6.61
CHEMBL4755269 IC50 = 275.0 nM 6.56
CHEMBL4749536 IC50 = 330.0 nM 6.48
CHEMBL4742925 IC50 = 530.0 nM 6.28
CHEMBL4754975 IC50 = 940.0 nM 6.03
CHEMBL4796191 IC50 = 960.0 nM 6.02
CHEMBL4748904 IC50 = 1700.0 nM 5.77
CHEMBL4792176 IC50 = 1900.0 nM 5.72
CHEMBL4747722 IC50 = 1900.0 nM 5.72
CHEMBL4760159 IC50 = 1900.0 nM 5.72
CHEMBL4759690 IC50 = 2120.0 nM 5.67
CHEMBL4756852 IC50 = 2310.0 nM 5.64
CHEMBL4777887 IC50 = 2700.0 nM 5.57
CHEMBL4746832 IC50 = 4000.0 nM 5.40
CHEMBL4798873 IC50 = 4800.0 nM 5.32
CHEMBL4763926 IC50 = 7900.0 nM 5.10
CHEMBL4794041 IC50 = 9800.0 nM 5.01
CHEMBL4780739 IC50 = 10000.0 nM 5.00