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Assay Detail

CHEMBL1930622

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of iNOS-mediated nitric oxide production in LPS-stimulated mouse BV2 cells measured after 24 hrs of post-stimulation by Griess reaction method
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type BV-2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase, inducible (CHEMBL3464)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Bis-chalcone analogues as potent NO production inhibitors and as cytotoxic agents.
Vijaya Bhaskar Reddy M, Shen YC, Ohkoshi E, Bastow KF, Qian K, Lee KH, Wu TS.
Eur J Med Chem (2012) 47 : 97 -103
PubMed 22115618 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 5.03 6.02

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1928925 1 6.02
CHEMBL1928924 1 5.74
CHEMBL1928928 1 5.40
CHEMBL1928921 1 5.26
CHEMBL1928926 1 5.15
CHEMBL1928923 1 5.09
CHEMBL1929082 1 5.06
CHEMBL7890 L-NAME 2.0 1 4.88
CHEMBL1929083 1 4.75
CHEMBL1928927 1 4.63
CHEMBL1928922 1 4.59
CHEMBL1926708 1 4.44
CHEMBL1928929 1 4.36
CHEMBL1929084 1 -
CHEMBL1929085 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1928925 IC50 = 950.0 nM 6.02
CHEMBL1928924 IC50 = 1820.0 nM 5.74
CHEMBL1928928 IC50 = 4000.0 nM 5.40
CHEMBL1928921 IC50 = 5440.0 nM 5.26
CHEMBL1928926 IC50 = 7040.0 nM 5.15
CHEMBL1928923 IC50 = 8090.0 nM 5.09
CHEMBL1929082 IC50 = 8650.0 nM 5.06
CHEMBL7890 L-NAME IC50 = 13350.0 nM 4.88
CHEMBL1929083 IC50 = 17740.0 nM 4.75
CHEMBL1928927 IC50 = 23290.0 nM 4.63
CHEMBL1928922 IC50 = 25690.0 nM 4.59
CHEMBL1926708 IC50 = 36310.0 nM 4.44
CHEMBL1928929 IC50 = 43270.0 nM 4.36
CHEMBL1929084 IC50 - -
CHEMBL1929085 IC50 - -