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Assay Detail

CHEMBL1930657

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of chimeric rat-human expressed in human HEK293 cells assessed as accumulation of [3H]cAMP after 1 hr by liquid scintillation counting
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HEK293
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Trace amine-associated receptor 1 is a stereoselective binding site for compounds in the amphetamine class.
Lewin AH, Miller GM, Gilmour B.
Bioorg Med Chem (2011) 19 : 7044 -7048
PubMed 22037049 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 5.46 5.95

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL612 DEXTROAMPHETAMINE 4.0 1 5.95
CHEMBL19393 LEVAMFETAMINE 4.0 1 5.51
CHEMBL1201201 METHAMPHETAMINE 4.0 1 5.35
CHEMBL1927030 LEVMETAMFETAMINE 2.0 1 5.01
CHEMBL1927024 (S)-P-HYDROXYAMPHETAMINE 1 -
CHEMBL1230845 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL612 DEXTROAMPHETAMINE EC50 = 1120.0 nM 5.95
CHEMBL19393 LEVAMFETAMINE EC50 = 3090.0 nM 5.51
CHEMBL1201201 METHAMPHETAMINE EC50 = 4440.0 nM 5.35
CHEMBL1927030 LEVMETAMFETAMINE EC50 = 9830.0 nM 5.01
CHEMBL1927024 (S)-P-HYDROXYAMPHETAMINE EC50 > 5420.0 nM -
CHEMBL1230845 EC50 > 8540.0 nM -