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Assay Detail

CHEMBL1935855

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR phosphorylation in EGF-stimulated human A431 after 2 hrs by Western blot analysis
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of novel 5-alkynyl-4-anilinopyrimidines as potent, orally active dual inhibitors of EGFR and Her-2 tyrosine kinases.
Suzuki N, Shiota T, Watanabe F, Haga N, Murashi T, Ohara T, Matsuo K, Omori N, Yari H, Dohi K, Inoue M, Iguchi M, Sentou J, Wada T.
Bioorg Med Chem Lett (2012) 22 : 456 -460
PubMed 22101132 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 8.67 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL553 ERLOTINIB 4.0 1 9.00
CHEMBL939 GEFITINIB 4.0 1 9.00
CHEMBL1201179 LAPATINIB DITOSYLATE 4.0 1 8.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL553 ERLOTINIB IC50 = 1.0 nM 9.00
CHEMBL939 GEFITINIB IC50 = 1.0 nM 9.00
CHEMBL1201179 LAPATINIB DITOSYLATE IC50 = 10.0 nM 8.00