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Assay Detail

CHEMBL1948413

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]epibatidine from Lymnaea stagnalis acetylcholine binding protein expressed using baculoviral system after 1.5 hrs by scintillation counting
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Lymnaea stagnalis
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholine-binding protein (CHEMBL6084)
Type SINGLE PROTEIN
Organism Lymnaea stagnalis

Publication

Structure-based design, synthesis and structure-activity relationships of dibenzosuberyl- and benzoate-substituted tropines as ligands for acetylcholine-binding protein.
Edink E, Akdemir A, Jansen C, van Elk R, Zuiderveld O, de Kanter FJ, van Muijlwijk-Koezen JE, Smit AB, Leurs R, de Esch IJ.
Bioorg Med Chem Lett (2012) 22 : 1448 -1454
PubMed 22243960 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 26 6.29 7.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1947055 1 7.50
CHEMBL1946189 1 7.40
CHEMBL1947053 1 7.10
CHEMBL1947206 1 7.00
CHEMBL1946188 1 7.00
CHEMBL1947060 1 6.90
CHEMBL1947059 1 6.90
CHEMBL1794606 1 6.60
CHEMBL3 NICOTINE 4.0 1 6.50
CHEMBL1946187 1 6.50
CHEMBL1947062 1 6.40
CHEMBL1947054 1 6.40
CHEMBL1947056 1 6.20
CHEMBL122270 LOBELINE 2.0 1 6.20
CHEMBL1947049 1 6.10
CHEMBL1947058 1 6.10
CHEMBL1947061 1 6.10
CHEMBL1946185 1 6.00
CHEMBL1947057 1 5.90
CHEMBL1947051 BENZOYL TROPINE 1 5.70
CHEMBL1947048 1 5.70
CHEMBL1947047 1 5.70
CHEMBL1946186 DEPTROPINE 2.0 1 5.70
CHEMBL1947205 1 5.70
CHEMBL1947052 1 5.20
CHEMBL1947050 1 5.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1947055 Ki = 31.62 nM 7.50
CHEMBL1946189 Ki = 39.81 nM 7.40
CHEMBL1947053 Ki = 79.43 nM 7.10
CHEMBL1947206 Ki = 100.0 nM 7.00
CHEMBL1946188 Ki = 100.0 nM 7.00
CHEMBL1947060 Ki = 125.89 nM 6.90
CHEMBL1947059 Ki = 125.89 nM 6.90
CHEMBL1794606 Ki = 251.19 nM 6.60
CHEMBL3 NICOTINE Ki = 316.23 nM 6.50
CHEMBL1946187 Ki = 316.23 nM 6.50
CHEMBL1947062 Ki = 398.11 nM 6.40
CHEMBL1947054 Ki = 398.11 nM 6.40
CHEMBL1947056 Ki = 630.96 nM 6.20
CHEMBL122270 LOBELINE Ki = 630.96 nM 6.20
CHEMBL1947049 Ki = 794.33 nM 6.10
CHEMBL1947058 Ki = 794.33 nM 6.10
CHEMBL1947061 Ki = 794.33 nM 6.10
CHEMBL1946185 Ki = 1000.0 nM 6.00
CHEMBL1947057 Ki = 1258.93 nM 5.90
CHEMBL1947051 BENZOYL TROPINE Ki = 1995.26 nM 5.70
CHEMBL1947048 Ki = 1995.26 nM 5.70
CHEMBL1947047 Ki = 1995.26 nM 5.70
CHEMBL1946186 DEPTROPINE Ki = 1995.26 nM 5.70
CHEMBL1947205 Ki = 1995.26 nM 5.70
CHEMBL1947052 Ki = 6309.57 nM 5.20
CHEMBL1947050 Ki = 7943.28 nM 5.10