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Assay Detail

CHEMBL1948717

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of dihydroorotate dehydrogenase in Wistar rat liver mitochondrial/microsomal membranes measured for 5 mins by 2,6-dichlorophenolindophenol reduction-based spectrophotometry
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dihydroorotate dehydrogenase (quinone), mitochondrial (CHEMBL2383)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

New inhibitors of dihydroorotate dehydrogenase (DHODH) based on the 4-hydroxy-1,2,5-oxadiazol-3-yl (hydroxyfurazanyl) scaffold.
Lolli ML, Giorgis M, Tosco P, Foti A, Fruttero R, Gasco A.
Eur J Med Chem (2012) 49 : 102 -109
PubMed 22245049 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 6.02 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL973 TERIFLUNOMIDE 4.0 1 7.70
CHEMBL38434 BREQUINAR 2.0 1 7.48
CHEMBL1946851 1 7.30
CHEMBL1946659 1 7.18
CHEMBL1946850 1 6.92
CHEMBL1946849 1 6.31
CHEMBL1946658 1 6.06
CHEMBL1946660 1 6.06
CHEMBL1946848 1 5.64
CHEMBL1946653 1 5.37
CHEMBL1946654 1 4.96
CHEMBL1946657 1 4.60
CHEMBL1946655 1 4.35
CHEMBL1946656 1 4.31

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL973 TERIFLUNOMIDE IC50 = 20.0 nM 7.70
CHEMBL38434 BREQUINAR IC50 = 33.0 nM 7.48
CHEMBL1946851 IC50 = 50.0 nM 7.30
CHEMBL1946659 IC50 = 66.0 nM 7.18
CHEMBL1946850 IC50 = 120.0 nM 6.92
CHEMBL1946849 IC50 = 490.0 nM 6.31
CHEMBL1946658 IC50 = 880.0 nM 6.06
CHEMBL1946660 IC50 = 870.0 nM 6.06
CHEMBL1946848 IC50 = 2300.0 nM 5.64
CHEMBL1946653 IC50 = 4300.0 nM 5.37
CHEMBL1946654 IC50 = 11000.0 nM 4.96
CHEMBL1946657 IC50 = 25000.0 nM 4.60
CHEMBL1946655 IC50 = 45000.0 nM 4.35
CHEMBL1946656 IC50 = 49000.0 nM 4.31