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Assay Detail

CHEMBL1960290

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Functional
Agonist activity at human adrenoceptor aplha 2B expressed in CHO cells assessed as rate of extracellular acidification by cytosensor microphysiometric analysis
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Alpha-2B adrenergic receptor (CHEMBL1942)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Might the observed α(2A)-adrenoreceptor agonism or antagonism of allyphenyline analogues be ascribed to different molecular conformations?
Diamanti E, Del Bello F, Carbonara G, Carrieri A, Fracchiolla G, Giannella M, Mammoli V, Piergentili A, Pohjanoksa K, Quaglia W, Scheinin M, Pigini M.
Bioorg Med Chem (2012) 20 : 2082 -2090
PubMed 22341244 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 20 5.91 6.90

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL17860 LOFEXIDINE 4.0 1 6.90
CHEMBL1956191 1 6.79
CHEMBL1956203 1 6.70
CHEMBL1956193 1 6.62
CHEMBL266613 1 6.60
CHEMBL1956202 1 6.53
CHEMBL1956192 1 6.50
CHEMBL1956201 1 6.50
CHEMBL157206 1 6.30
CHEMBL157955 1 6.00
CHEMBL155866 1 5.83
CHEMBL1956194 1 5.80
CHEMBL14208 1 5.76
CHEMBL1956198 1 5.50
CHEMBL1956196 1 5.50
CHEMBL1956195 CYCLOMETHYLINE 1 5.48
CHEMBL1956200 1 5.00
CHEMBL156336 1 5.00
CHEMBL1956197 1 4.89
CHEMBL1956199 1 4.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL17860 LOFEXIDINE EC50 = 125.89 nM 6.90
CHEMBL1956191 EC50 = 162.18 nM 6.79
CHEMBL1956203 EC50 = 199.53 nM 6.70
CHEMBL1956193 EC50 = 239.88 nM 6.62
CHEMBL266613 EC50 = 251.19 nM 6.60
CHEMBL1956202 EC50 = 295.12 nM 6.53
CHEMBL1956192 EC50 = 316.23 nM 6.50
CHEMBL1956201 EC50 = 316.23 nM 6.50
CHEMBL157206 EC50 = 501.19 nM 6.30
CHEMBL157955 EC50 = 1000.0 nM 6.00
CHEMBL155866 EC50 = 1479.11 nM 5.83
CHEMBL1956194 EC50 = 1584.89 nM 5.80
CHEMBL14208 EC50 = 1737.8 nM 5.76
CHEMBL1956198 EC50 = 3162.28 nM 5.50
CHEMBL1956196 EC50 = 3162.28 nM 5.50
CHEMBL1956195 CYCLOMETHYLINE EC50 = 3311.31 nM 5.48
CHEMBL1956200 EC50 = 10000.0 nM 5.00
CHEMBL156336 EC50 = 10000.0 nM 5.00
CHEMBL1956197 EC50 = 12882.5 nM 4.89
CHEMBL1956199 EC50 = 100000.0 nM 4.00