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Assay Detail

CHEMBL1961032

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of CDC7/DBF4-mediated MCM2 phosphorylation in human HCT116 cells after 6 hrs by spectrophotometric analysis
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HCT-116
Confidence 7 — Homologous single protein target
Curated By Autocuration

Publication

Aminopyrimidinone cdc7 kinase inhibitors.
Woods KW, Lai C, Miyashiro JM, Tong Y, Florjancic AS, Han EK, Soni N, Shi Y, Lasko L, Leverson JD, Johnson EF, Shoemaker AR, Penning TD.
Bioorg Med Chem Lett (2012) 22 : 1940 -1943
PubMed 22326396 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 12 6.65 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1958411 1 7.82
CHEMBL1958405 1 7.22
CHEMBL1958414 1 7.22
CHEMBL1958412 1 6.84
CHEMBL1958413 1 6.75
CHEMBL1958415 1 6.52
CHEMBL1958416 1 6.52
CHEMBL1958420 1 6.52
CHEMBL1958418 1 6.39
CHEMBL1958419 1 5.75
CHEMBL1958417 1 5.58
CHEMBL1958421 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1958411 EC50 = 15.0 nM 7.82
CHEMBL1958405 EC50 = 60.0 nM 7.22
CHEMBL1958414 EC50 = 60.0 nM 7.22
CHEMBL1958412 EC50 = 143.0 nM 6.84
CHEMBL1958413 EC50 = 180.0 nM 6.75
CHEMBL1958415 EC50 = 300.0 nM 6.52
CHEMBL1958416 EC50 = 300.0 nM 6.52
CHEMBL1958420 EC50 = 300.0 nM 6.52
CHEMBL1958418 EC50 = 410.0 nM 6.39
CHEMBL1958419 EC50 = 1780.0 nM 5.75
CHEMBL1958417 EC50 = 2610.0 nM 5.58
CHEMBL1958421 EC50 - -