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Assay Detail

CHEMBL2013287

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of steroid sulfatase in human MCF7 cells using [3H]E1S as substrate after 20 hrs by scintillation spectrometry
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type MCF7
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Steryl-sulfatase (CHEMBL3559)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and evaluation of analogues of estrone-3-O-sulfamate as potent steroid sulfatase inhibitors.
Lawrence Woo LW, Leblond B, Purohit A, Potter BV.
Bioorg Med Chem (2012) 20 : 2506 -2519
PubMed 22455789 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 8.74 11.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2011408 1 11.00
CHEMBL364332 1 9.96
CHEMBL2011415 1 9.36
CHEMBL2011414 1 9.21
CHEMBL364745 1 9.09
CHEMBL122708 EMATE 1 9.08
CHEMBL2011416 1 8.92
CHEMBL2011407 1 8.08
CHEMBL2011409 1 7.43
CHEMBL2011417 1 7.38
CHEMBL2011411 1 6.63
CHEMBL2011419 1 -
CHEMBL2011420 1 -
CHEMBL2011421 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2011408 IC50 = 0.01 nM 11.00
CHEMBL364332 IC50 = 0.11 nM 9.96
CHEMBL2011415 IC50 = 0.44 nM 9.36
CHEMBL2011414 IC50 = 0.62 nM 9.21
CHEMBL364745 IC50 = 0.81 nM 9.09
CHEMBL122708 EMATE IC50 = 0.83 nM 9.08
CHEMBL2011416 IC50 = 1.2 nM 8.92
CHEMBL2011407 IC50 = 8.3 nM 8.08
CHEMBL2011409 IC50 = 37.0 nM 7.43
CHEMBL2011417 IC50 = 42.0 nM 7.38
CHEMBL2011411 IC50 = 236.0 nM 6.63
CHEMBL2011419 IC50 - -
CHEMBL2011420 IC50 - -
CHEMBL2011421 IC50 - -