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Assay Detail

CHEMBL2014114

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminal GST-tagged DYRK2 expressed in baculovirus using DYRKtide-F as substrate assessed as inhibition of [33P] incorporation into substrate using [gamma33P]ATP after 10 mins by liquid scintillation counting
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Structure-activity relationship study of beta-carboline derivatives as haspin kinase inhibitors.
Cuny GD, Ulyanova NP, Patnaik D, Liu JF, Lin X, Auerbach K, Ray SS, Xian J, Glicksman MA, Stein RL, Higgins JM.
Bioorg Med Chem Lett (2012) 22 : 2015 -2019
PubMed 22335895 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 5.13 5.32

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2012570 1 5.32
CHEMBL2012568 1 5.31
CHEMBL2012567 1 5.16
CHEMBL2012571 1 5.10
CHEMBL2012575 1 5.07
CHEMBL2012574 1 4.82
CHEMBL2012558 1 -
CHEMBL2012573 1 -
CHEMBL2012572 1 -
CHEMBL2012559 1 -
CHEMBL2012560 1 -
CHEMBL2012569 1 -
CHEMBL2012561 1 -
CHEMBL2012562 1 -
CHEMBL2012566 1 -
CHEMBL2012565 1 -
CHEMBL2012564 1 -
CHEMBL2012563 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2012570 IC50 = 4800.0 nM 5.32
CHEMBL2012568 IC50 = 4900.0 nM 5.31
CHEMBL2012567 IC50 = 7000.0 nM 5.16
CHEMBL2012571 IC50 = 8000.0 nM 5.10
CHEMBL2012575 IC50 = 8500.0 nM 5.07
CHEMBL2012574 IC50 = 15000.0 nM 4.82
CHEMBL2012558 IC50 > 10000.0 nM -
CHEMBL2012573 IC50 > 10000.0 nM -
CHEMBL2012572 IC50 > 10000.0 nM -
CHEMBL2012559 IC50 > 10000.0 nM -
CHEMBL2012560 IC50 > 10000.0 nM -
CHEMBL2012569 IC50 > 10000.0 nM -
CHEMBL2012561 IC50 > 10000.0 nM -
CHEMBL2012562 IC50 > 10000.0 nM -
CHEMBL2012566 IC50 > 10000.0 nM -
CHEMBL2012565 IC50 > 10000.0 nM -
CHEMBL2012564 IC50 > 10000.0 nM -
CHEMBL2012563 IC50 > 10000.0 nM -