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Assay Detail

CHEMBL2014329

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Transactivation of GAL4-fused PPARalpha LBD expressed in HepG2 cells after 20 hrs by luminescence assay
13
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HepG2
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Peroxisome proliferator-activated receptor alpha (CHEMBL239)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Anti-inflammatory and PPAR transactivational effects of secondary metabolites from the roots of Asarum sieboldii.
Quang TH, Ngan NT, Minh CV, Kiem PV, Tai BH, Thao NP, Song SB, Kim YH.
Bioorg Med Chem Lett (2012) 22 : 2527 -2533
PubMed 22381047 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 10 4.84 6.05
Activity 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL557555 CIPROFIBRATE 4.0 1 6.05
CHEMBL2011544 2 5.40
CHEMBL2011543 2 4.80
CHEMBL2011541 2 4.71
CHEMBL2011545 1 4.34
CHEMBL108861 METHYLEUGENOL 1 4.33
CHEMBL2011540 PIPERITOL 1 4.27
CHEMBL2011538 1 -
CHEMBL2011539 PLUVIATILOL 1 -
CHEMBL487611 PINORESINOL 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL557555 CIPROFIBRATE EC50 = 900.0 nM 6.05
CHEMBL2011544 EC50 = 4000.0 nM 5.40
CHEMBL2011543 EC50 = 15700.0 nM 4.80
CHEMBL2011541 EC50 = 19500.0 nM 4.71
CHEMBL2011545 EC50 = 46100.0 nM 4.34
CHEMBL108861 METHYLEUGENOL EC50 = 46500.0 nM 4.33
CHEMBL2011540 PIPERITOL EC50 = 53200.0 nM 4.27
CHEMBL2011538 EC50 > 60000.0 nM -
CHEMBL2011539 PLUVIATILOL EC50 > 60000.0 nM -
CHEMBL487611 PINORESINOL EC50 > 60000.0 nM -
CHEMBL2011541 Activity - -
CHEMBL2011543 Activity - -
CHEMBL2011544 Activity - -