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Assay Detail

CHEMBL2015672

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant VEGF-R2 expressed in baculovirus expression system using GST-PLCgamma as substrate after 1 hr by time-resolved fluorescence analysis
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Vascular endothelial growth factor receptor 2 (CHEMBL279)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological profile of the pan-vascular endothelial growth factor receptor/tyrosine kinase with immunoglobulin and epidermal growth factor-like homology domains 2 (VEGF-R/TIE-2) inhibitor 11-(2-methylpropyl)-12,13-dihydro-2-methyl-8-(pyrimidin-2-ylamino)-4H-indazolo[5,4-a]pyrrolo[3,4-c]carbazol-4-one (CEP-11981): a novel oncology therapeutic agent.
Hudkins RL, Becknell NC, Zulli AL, Underiner TL, Angeles TS, Aimone LD, Albom MS, Chang H, Miknyoczki SJ, Hunter K, Jones-Bolin S, Zhao H, Bacon ER, Mallamo JP, Ator MA, Ruggeri BA.
J Med Chem (2012) 55 : 903 -913
PubMed 22148921 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.69 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2010870 1 8.52
CHEMBL2010872 CEP-11981 2.0 1 8.40
CHEMBL2010871 1 8.22
CHEMBL2010869 1 8.05
CHEMBL2010877 1 8.00
CHEMBL2010873 1 7.85
CHEMBL2010875 1 7.50
CHEMBL2011061 1 7.20
CHEMBL2010878 1 6.59
CHEMBL2010876 1 6.57
CHEMBL2010874 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2010870 IC50 = 3.0 nM 8.52
CHEMBL2010872 CEP-11981 IC50 = 4.0 nM 8.40
CHEMBL2010871 IC50 = 6.0 nM 8.22
CHEMBL2010869 IC50 = 9.0 nM 8.05
CHEMBL2010877 IC50 = 10.0 nM 8.00
CHEMBL2010873 IC50 = 14.0 nM 7.85
CHEMBL2010875 IC50 = 32.0 nM 7.50
CHEMBL2011061 IC50 = 63.0 nM 7.20
CHEMBL2010878 IC50 = 256.0 nM 6.59
CHEMBL2010876 IC50 = 271.0 nM 6.57
CHEMBL2010874 IC50 > 3000.0 nM -