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Assay Detail

CHEMBL2020707

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of porcine pancreatic lipase using p-nitrophenylbutyrate as substrate preincubated for 15 mins prior substrate addition measured after 15 mins by spectrophotometry
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Sus scrofa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Pancreatic triacylglycerol lipase (CHEMBL1687677)
Type SINGLE PROTEIN
Organism Sus scrofa

Publication

A new pancreatic lipase inhibitor from Broussonetia kanzinoki.
Ahn JH, Liu Q, Lee C, Ahn MJ, Yoo HS, Hwang BY, Lee MK.
Bioorg Med Chem Lett (2012) 22 : 2760 -2763
PubMed 22450131 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 4.65 6.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL175247 ORLISTAT 4.0 1 6.52
CHEMBL2017114 BROUSSONONE A 1 4.55
CHEMBL463454 BROUSSONIN B 1 4.12
CHEMBL508033 1 4.07
CHEMBL465879 BROUSSONIN A 1 4.01
CHEMBL2017115 1 -
CHEMBL2017116 1 -
CHEMBL2017117 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL175247 ORLISTAT IC50 = 300.0 nM 6.52
CHEMBL2017114 BROUSSONONE A IC50 = 28400.0 nM 4.55
CHEMBL463454 BROUSSONIN B IC50 = 75400.0 nM 4.12
CHEMBL508033 IC50 = 85100.0 nM 4.07
CHEMBL465879 BROUSSONIN A IC50 = 98700.0 nM 4.01
CHEMBL2017115 IC50 > 100000.0 nM -
CHEMBL2017116 IC50 > 100000.0 nM -
CHEMBL2017117 IC50 > 100000.0 nM -