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Assay Detail

CHEMBL2025481

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against CCR5-tropic HIV1 NL-AD87 infected in HEK293T cells assessed as inhibition of viral replication after 4 days by beta-galactosidase reporter gene assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Human immunodeficiency virus 1
Cell Type HEK-293T
Confidence 1 — Organism
Curated By Autocuration

Target

Human immunodeficiency virus 1 (CHEMBL378)
Type ORGANISM
Organism Human immunodeficiency virus 1

Publication

Library-based discovery and characterization of daphnane diterpenes as potent and selective HIV inhibitors in Daphne gnidium.
Vidal V, Potterat O, Louvel S, Hamy F, Mojarrab M, Sanglier JJ, Klimkait T, Hamburger M.
J Nat Prod (2012) 75 : 414 -419
PubMed 22148316 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 7.00 7.75

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2023370 GNIDICIN 1 7.75
CHEMBL2023369 DAPHNETOXIN 1 7.57
CHEMBL1159653 GNIDITRIN 1 6.88
CHEMBL447856 EXCOECARIA TOXIN 1 5.80

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2023370 GNIDICIN EC50 = 17.6 nM 7.75
CHEMBL2023369 DAPHNETOXIN EC50 = 26.7 nM 7.57
CHEMBL1159653 GNIDITRIN EC50 = 133.0 nM 6.88
CHEMBL447856 EXCOECARIA TOXIN EC50 = 1567.0 nM 5.80