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Assay Detail

CHEMBL2027168

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human 11beta-HSD2 expressed in HEK293 cells assessed as [3H]cortisol production after 60 mins by SPA
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

11-beta-hydroxysteroid dehydrogenase type 2 (CHEMBL3746)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and evaluation of piperidine urea derivatives as efficacious 11β-hydroxysteroid dehydrogenase type 1 inhibitors in diabetic ob/ob mice.
Zhang L, Chen J, Ning M, Zou Q, Leng Y, Shen J.
Bioorg Med Chem Lett (2012) 22 : 2748 -2752
PubMed 22440625 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 6.72 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL441687 GLYCYRRHIZIN 2.0 1 9.00
CHEMBL2023587 1 6.14
CHEMBL2023585 1 6.07
CHEMBL2023584 1 5.66

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL441687 GLYCYRRHIZIN EC50 = 1.0 nM 9.00
CHEMBL2023587 EC50 = 720.0 nM 6.14
CHEMBL2023585 EC50 = 860.0 nM 6.07
CHEMBL2023584 EC50 = 2200.0 nM 5.66