Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL2033031

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of Smo-mediated Hh signaling in human Shh-light2 cells by luciferase reporter gene assay
ChEMBL ↗
68
Total Activities
68
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type Shh Light II
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Protein smoothened (CHEMBL5971) ↗
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Acylthiourea, acylurea, and acylguanidine derivatives with potent hedgehog inhibiting activity.
Solinas A, Faure H, Roudaut H, Traiffort E, Schoenfelder A, Mann A, Manetti F, Taddei M, Ruat M.
J Med Chem (2012) 55 : 1559 -1571
PubMed 22268551 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 68 6.87 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL473417 VISMODEGIB 4.0 1 8.15
CHEMBL2031277 — — 1 7.89
CHEMBL2031288 — — 1 7.85
CHEMBL2031290 — — 1 7.82
CHEMBL2031263 — — 1 7.54
CHEMBL2031287 — — 1 7.48
CHEMBL2031268 — — 1 7.39
CHEMBL2031289 — — 1 7.16
CHEMBL2031283 — — 1 7.16
CHEMBL2031077 — — 1 7.10
CHEMBL2031098 — — 1 7.05
CHEMBL2031245 — — 1 7.00
CHEMBL2031291 — — 1 6.96
CHEMBL2031281 — — 1 6.96
CHEMBL2031267 — — 1 6.89
CHEMBL480889 — — 1 6.85
CHEMBL2031273 — — 1 6.80
CHEMBL2031295 — — 1 6.80
CHEMBL2031253 — — 1 6.75
CHEMBL2031294 — — 1 6.72
CHEMBL2031258 — — 1 6.72
CHEMBL2031284 — — 1 6.66
CHEMBL2031282 — — 1 6.54
CHEMBL254129 CYCLOPAMINE — 1 6.52
CHEMBL2031292 — — 1 6.43
CHEMBL2031080 — — 1 6.28
CHEMBL2031285 — — 1 6.19
CHEMBL2031084 — — 1 6.19
CHEMBL2031254 — — 1 6.09
CHEMBL2031286 — — 1 6.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL473417 VISMODEGIB IC50 = 7.0 nM 8.15
CHEMBL2031277 — IC50 = 13.0 nM 7.89
CHEMBL2031288 — IC50 = 14.0 nM 7.85
CHEMBL2031290 — IC50 = 15.0 nM 7.82
CHEMBL2031263 — IC50 = 29.0 nM 7.54
CHEMBL2031287 — IC50 = 33.0 nM 7.48
CHEMBL2031268 — IC50 = 41.0 nM 7.39
CHEMBL2031289 — IC50 = 70.0 nM 7.16
CHEMBL2031283 — IC50 = 70.0 nM 7.16
CHEMBL2031077 — IC50 = 80.0 nM 7.10
CHEMBL2031098 — IC50 = 90.0 nM 7.05
CHEMBL2031245 — IC50 = 100.0 nM 7.00
CHEMBL2031291 — IC50 = 110.0 nM 6.96
CHEMBL2031281 — IC50 = 110.0 nM 6.96
CHEMBL2031267 — IC50 = 130.0 nM 6.89
CHEMBL480889 — IC50 = 140.0 nM 6.85
CHEMBL2031273 — IC50 = 160.0 nM 6.80
CHEMBL2031295 — IC50 = 160.0 nM 6.80
CHEMBL2031253 — IC50 = 180.0 nM 6.75
CHEMBL2031294 — IC50 = 190.0 nM 6.72
CHEMBL2031258 — IC50 = 190.0 nM 6.72
CHEMBL2031284 — IC50 = 220.0 nM 6.66
CHEMBL2031282 — IC50 = 290.0 nM 6.54
CHEMBL254129 CYCLOPAMINE IC50 = 300.0 nM 6.52
CHEMBL2031292 — IC50 = 370.0 nM 6.43
CHEMBL2031080 — IC50 = 520.0 nM 6.28
CHEMBL2031084 — IC50 = 650.0 nM 6.19
CHEMBL2031285 — IC50 = 640.0 nM 6.19
CHEMBL2031254 — IC50 = 820.0 nM 6.09
CHEMBL2031286 — IC50 = 900.0 nM 6.05
CHEMBL2031256 — IC50 = 1100.0 nM 5.96
CHEMBL2031079 — IC50 = 1800.0 nM 5.75
CHEMBL2031096 — IC50 - — -
CHEMBL2031097 — IC50 - — -
CHEMBL2031244 — IC50 - — -
CHEMBL2031081 — IC50 - — -
CHEMBL2031246 — IC50 - — -
CHEMBL2031257 — IC50 - — -
CHEMBL2031248 — IC50 - — -
CHEMBL2031082 — IC50 - — -
CHEMBL2031249 — IC50 - — -
CHEMBL2031250 — IC50 - — -
CHEMBL2031251 — IC50 - — -
CHEMBL2031252 — IC50 - — -
CHEMBL2031083 — IC50 - — -
CHEMBL2031255 — IC50 - — -
CHEMBL2031247 — IC50 - — -
CHEMBL2031279 — IC50 - — -
CHEMBL2031280 — IC50 - — -
CHEMBL2031278 — IC50 - — -