Assay Detail
Binding
CHEMBL2033036
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of BODIPY-labelled cyclopamine from human Smo receptor expressed in HEK293 cells after 2 hrs by fluorescence microscopy
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Acylthiourea, acylurea, and acylguanidine derivatives with potent hedgehog inhibiting activity.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 7.42 | 8.30 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2031290 | — | — | 1 | 8.30 |
| CHEMBL473417 | VISMODEGIB | 4.0 | 1 | 8.15 |
| CHEMBL2031277 | — | — | 1 | 7.62 |
| CHEMBL2031268 | — | — | 1 | 7.20 |
| CHEMBL254129 | CYCLOPAMINE | — | 1 | 7.19 |
| CHEMBL2031273 | — | — | 1 | 6.97 |
| CHEMBL2031084 | — | — | 1 | 6.52 |
| CHEMBL480889 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2031290 | — | IC50 | = | 5.0 | nM | 8.30 |
| CHEMBL473417 | VISMODEGIB | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL2031277 | — | IC50 | = | 24.0 | nM | 7.62 |
| CHEMBL2031268 | — | IC50 | = | 63.0 | nM | 7.20 |
| CHEMBL254129 | CYCLOPAMINE | IC50 | = | 64.0 | nM | 7.19 |
| CHEMBL2031273 | — | IC50 | = | 107.0 | nM | 6.97 |
| CHEMBL2031084 | — | IC50 | = | 300.0 | nM | 6.52 |
| CHEMBL480889 | — | IC50 | - | — | - |