Assay Detail
Functional
CHEMBL2033581
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inverse agonist activity at PPARbeta/delta in mouse C2C12 cells assessed as inhibition of Angptl4 expression after 24 hrs by RT-qPCR analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Mus musculus |
| Cell Type | C2C12 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Peroxisome proliferator-activated receptor delta (CHEMBL2458) ↗| Type | SINGLE PROTEIN |
| Organism | Mus musculus |
Publication
(Z)-2-(2-bromophenyl)-3-{[4-(1-methyl-piperazine)amino]phenyl}acrylonitrile (DG172): an orally bioavailable PPARβ/δ-selective ligand with inverse agonistic properties.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 7.54 | 8.02 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2030550 | — | — | 1 | 8.02 |
| CHEMBL592652 | — | — | 1 | 7.32 |
| CHEMBL2030543 | — | — | 1 | 7.28 |
| CHEMBL1998001 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2030550 | — | IC50 | = | 9.5 | nM | 8.02 |
| CHEMBL592652 | — | IC50 | = | 48.0 | nM | 7.32 |
| CHEMBL2030543 | — | IC50 | = | 52.0 | nM | 7.28 |
| CHEMBL1998001 | — | IC50 | > | 500.0 | nM | - |