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Assay Detail

CHEMBL2034299

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of PDK1 using [gamma-33P]-ATP as substrate after 30 mins by scintillation counting
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

3-phosphoinositide-dependent protein kinase 1 (CHEMBL2534)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Thieno[3,2-d]pyrimidin-4(3H)-one derivatives as PDK1 inhibitors discovered by fragment-based screening.
Lee AC, Ramanujulu PM, Poulsen A, Williams M, Blanchard S, Ma DM, Bonday Z, Goh KL, Goh KC, Goh MK, Wood J, Dymock BW.
Bioorg Med Chem Lett (2012) 22 : 4023 -4027
PubMed 22591730 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 22 3.97 5.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2029526 1 5.28
CHEMBL2029524 1 4.96
CHEMBL2029521 1 4.82
CHEMBL2029523 1 4.48
CHEMBL2029525 1 4.41
CHEMBL2029511 1 4.26
CHEMBL2029513 1 4.21
CHEMBL1612732 1 4.07
CHEMBL271138 1 4.04
CHEMBL2029520 1 4.02
CHEMBL2029522 1 3.98
CHEMBL2029519 1 3.96
CHEMBL2029517 1 3.91
CHEMBL2029518 1 3.81
CHEMBL2029516 1 3.56
CHEMBL23507 5-FLUOROINDOLE-2-CARBOXYLIC ACID 1 3.53
CHEMBL2029514 1 3.52
CHEMBL2029515 1 3.40
CHEMBL1877245 1 3.34
CHEMBL2029512 1 3.33
CHEMBL1891423 1 3.31
CHEMBL2029510 1 3.08

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2029526 IC50 = 5200.0 nM 5.28
CHEMBL2029524 IC50 = 11000.0 nM 4.96
CHEMBL2029521 IC50 = 15000.0 nM 4.82
CHEMBL2029523 IC50 = 33000.0 nM 4.48
CHEMBL2029525 IC50 = 39000.0 nM 4.41
CHEMBL2029511 IC50 = 55000.0 nM 4.26
CHEMBL2029513 IC50 = 61000.0 nM 4.21
CHEMBL1612732 IC50 = 86000.0 nM 4.07
CHEMBL271138 IC50 = 92000.0 nM 4.04
CHEMBL2029520 IC50 = 95000.0 nM 4.02
CHEMBL2029522 IC50 = 106000.0 nM 3.98
CHEMBL2029519 IC50 = 109000.0 nM 3.96
CHEMBL2029517 IC50 = 122000.0 nM 3.91
CHEMBL2029518 IC50 = 154000.0 nM 3.81
CHEMBL2029516 IC50 = 273000.0 nM 3.56
CHEMBL23507 5-FLUOROINDOLE-2-CARBOXYLIC ACID IC50 = 297000.0 nM 3.53
CHEMBL2029514 IC50 = 302000.0 nM 3.52
CHEMBL2029515 IC50 = 402000.0 nM 3.40
CHEMBL1877245 IC50 = 461000.0 nM 3.34
CHEMBL2029512 IC50 = 472000.0 nM 3.33
CHEMBL1891423 IC50 = 492000.0 nM 3.31
CHEMBL2029510 IC50 = 828000.0 nM 3.08