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Assay Detail

CHEMBL2039155

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant HPGDS using PGH2 as substrate assessed as production of PGD2 preincubated for 10 mins prior substrate addition measured after 42 secs by TBA-based fluorescence assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hematopoietic prostaglandin D synthase (CHEMBL5879)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Investigation of the binding pocket of human hematopoietic prostaglandin (PG) D2 synthase (hH-PGDS): a tale of two waters.
Trujillo JI, Kiefer JR, Huang W, Day JE, Moon J, Jerome GM, Bono CP, Kornmeier CM, Williams ML, Kuhn C, Rennie GR, Wynn TA, Carron CP, Thorarensen A.
Bioorg Med Chem Lett (2012) 22 : 3795 -3799
PubMed 22546671 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.89 8.63

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2035651 1 8.63
CHEMBL2035653 1 8.08
CHEMBL2035652 1 6.56
CHEMBL2035654 1 6.18
CHEMBL2035656 1 6.07
CHEMBL2035655 1 5.83

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2035651 IC50 = 2.34 nM 8.63
CHEMBL2035653 IC50 = 8.26 nM 8.08
CHEMBL2035652 IC50 = 274.0 nM 6.56
CHEMBL2035654 IC50 = 662.0 nM 6.18
CHEMBL2035656 IC50 = 845.0 nM 6.07
CHEMBL2035655 IC50 = 1480.0 nM 5.83