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Assay Detail

CHEMBL2049774

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FAAH in rat brain membranes assessed as hydrolysis of [14C]-anandamide preincubated for 20 mins before [14C]-anandamide addition measured after 30 mins
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Subcellular Brain membranes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fatty-acid amide hydrolase 1 (CHEMBL3229)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

The First Dual ChE/FAAH Inhibitors: New Perspectives for Alzheimer's Disease?
Rampa A, Bartolini M, Bisi A, Belluti F, Gobbi S, Andrisano V, Ligresti A, Di Marzo V.
ACS Med Chem Lett (2012) 3 : 182 -186
PubMed 24900454 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.65 8.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL184238 URB-597 1.0 1 8.22
CHEMBL2047530 1 7.40
CHEMBL2047528 1 7.30
CHEMBL336297 1 7.00
CHEMBL2047527 1 6.96
CHEMBL440526 1 6.77
CHEMBL2047526 1 6.54
CHEMBL128390 1 6.40
CHEMBL128043 1 6.10
CHEMBL130918 1 5.85
CHEMBL2047529 1 5.74
CHEMBL2047531 1 5.57
CHEMBL2047525 1 -
CHEMBL128551 1 -
CHEMBL132110 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL184238 URB-597 IC50 = 6.0 nM 8.22
CHEMBL2047530 IC50 = 40.0 nM 7.40
CHEMBL2047528 IC50 = 50.0 nM 7.30
CHEMBL336297 IC50 = 100.0 nM 7.00
CHEMBL2047527 IC50 = 110.0 nM 6.96
CHEMBL440526 IC50 = 170.0 nM 6.77
CHEMBL2047526 IC50 = 290.0 nM 6.54
CHEMBL128390 IC50 = 400.0 nM 6.40
CHEMBL128043 IC50 = 800.0 nM 6.10
CHEMBL130918 IC50 = 1400.0 nM 5.85
CHEMBL2047529 IC50 = 1820.0 nM 5.74
CHEMBL2047531 IC50 = 2710.0 nM 5.57
CHEMBL2047525 IC50 - -
CHEMBL128551 IC50 - -
CHEMBL132110 IC50 - -