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Assay Detail

CHEMBL2050879

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ABCG2-mediated mitoxantrone efflux expressed in HEK293 cells after 48 hrs by flow cytometric analysis
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HEK293
Confidence 8 — Homologous single protein target
Curated By Autocuration

Publication

Investigation of chalcones as selective inhibitors of the breast cancer resistance protein: critical role of methoxylation in both inhibition potency and cytotoxicity.
Valdameri G, Gauthier C, Terreux R, Kachadourian R, Day BJ, Winnischofer SM, Rocha ME, Frachet V, Ronot X, Di Pietro A, Boumendjel A.
J Med Chem (2012) 55 : 3193 -3200
PubMed 22449016 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 6.53 6.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL404782 1 6.77
CHEMBL406837 1 6.58
CHEMBL1834565 1 6.57
CHEMBL260654 1 6.46
CHEMBL1834568 1 6.46
CHEMBL1834566 1 6.35

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL404782 IC50 = 170.0 nM 6.77
CHEMBL406837 IC50 = 260.0 nM 6.58
CHEMBL1834565 IC50 = 270.0 nM 6.57
CHEMBL260654 IC50 = 350.0 nM 6.46
CHEMBL1834568 IC50 = 350.0 nM 6.46
CHEMBL1834566 IC50 = 450.0 nM 6.35