Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL2051182

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of [3H]m7-GTP binding to human FLAG-His6 tagged eIF4E expressed in Escherichia coli by scintillation proximity assay
26
Total Activities
26
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Eukaryotic translation initiation factor 4E (CHEMBL4848)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure-guided design, synthesis, and evaluation of guanine-derived inhibitors of the eIF4E mRNA-cap interaction.
Chen X, Kopecky DJ, Mihalic J, Jeffries S, Min X, Heath J, Deignan J, Lai S, Fu Z, Guimaraes C, Shen S, Li S, Johnstone S, Thibault S, Xu H, Cardozo M, Shen W, Walker N, Kayser F, Wang Z.
J Med Chem (2012) 55 : 3837 -3851
PubMed 22458568 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 5.80 7.80
Ki 1 7.55 7.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2046918 1 7.80
CHEMBL1094973 1 7.55
CHEMBL2046906 1 7.39
CHEMBL2046915 1 7.29
CHEMBL2046910 1 7.25
CHEMBL2046905 1 7.23
CHEMBL2046916 1 7.02
CHEMBL2046912 1 6.93
CHEMBL2046908 1 6.90
CHEMBL2046917 1 6.80
CHEMBL2046920 1 5.87
CHEMBL2046907 1 5.80
CHEMBL2046909 1 5.59
CHEMBL2046921 1 5.50
CHEMBL2046914 1 5.44
CHEMBL2046903 1 5.27
CHEMBL2046913 1 5.18
CHEMBL1095620 1 5.00
CHEMBL1097198 1 4.85
CHEMBL1094975 1 4.81
CHEMBL2046904 1 4.37
CHEMBL2046902 1 4.31
CHEMBL1095941 1 4.26
CHEMBL1097199 1 4.25
CHEMBL2046911 1 4.07
CHEMBL2046919 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2046918 IC50 = 16.0 nM 7.80
CHEMBL1094973 Ki = 28.0 nM 7.55
CHEMBL2046906 IC50 = 41.0 nM 7.39
CHEMBL2046915 IC50 = 51.0 nM 7.29
CHEMBL2046910 IC50 = 56.0 nM 7.25
CHEMBL2046905 IC50 = 59.0 nM 7.23
CHEMBL2046916 IC50 = 95.0 nM 7.02
CHEMBL2046912 IC50 = 117.0 nM 6.93
CHEMBL2046908 IC50 = 125.0 nM 6.90
CHEMBL2046917 IC50 = 160.0 nM 6.80
CHEMBL2046920 IC50 = 1350.0 nM 5.87
CHEMBL2046907 IC50 = 1590.0 nM 5.80
CHEMBL2046909 IC50 = 2570.0 nM 5.59
CHEMBL2046921 IC50 = 3140.0 nM 5.50
CHEMBL2046914 IC50 = 3600.0 nM 5.44
CHEMBL2046903 IC50 = 5350.0 nM 5.27
CHEMBL2046913 IC50 = 6580.0 nM 5.18
CHEMBL1095620 IC50 = 10000.0 nM 5.00
CHEMBL1097198 IC50 = 14000.0 nM 4.85
CHEMBL1094975 IC50 = 15500.0 nM 4.81
CHEMBL2046904 IC50 = 43000.0 nM 4.37
CHEMBL2046902 IC50 = 49300.0 nM 4.31
CHEMBL1095941 IC50 = 54500.0 nM 4.26
CHEMBL1097199 IC50 = 56800.0 nM 4.25
CHEMBL2046911 IC50 = 85400.0 nM 4.07
CHEMBL2046919 IC50 = 300000.0 nM -