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Assay Detail

CHEMBL2060147

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat DPP4 using Gly-Pro-MCA as substrate after 60 mins by fluorometry
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dipeptidyl peptidase 4 (CHEMBL4653)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Fused bicyclic heteroarylpiperazine-substituted L-prolylthiazolidines as highly potent DPP-4 inhibitors lacking the electrophilic nitrile group.
Yoshida T, Akahoshi F, Sakashita H, Sonda S, Takeuchi M, Tanaka Y, Nabeno M, Kishida H, Miyaguchi I, Hayashi Y.
Bioorg Med Chem (2012) 20 : 5033 -5041
PubMed 22824762 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 9.03 9.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2058975 1 9.25
CHEMBL2058971 1 9.23
CHEMBL2058762 1 9.21
CHEMBL2058972 1 9.21
CHEMBL2058758 1 9.16
CHEMBL2058756 1 9.14
CHEMBL2058757 1 9.11
CHEMBL2058974 1 9.10
CHEMBL2058973 1 9.08
CHEMBL2058969 1 9.00
CHEMBL2058761 1 8.99
CHEMBL2058760 1 8.96
CHEMBL399610 1 8.96
CHEMBL2058759 1 8.89
CHEMBL2058970 1 8.82
CHEMBL2058967 1 8.82
CHEMBL2058968 1 8.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2058975 IC50 = 0.56 nM 9.25
CHEMBL2058971 IC50 = 0.59 nM 9.23
CHEMBL2058762 IC50 = 0.61 nM 9.21
CHEMBL2058972 IC50 = 0.62 nM 9.21
CHEMBL2058758 IC50 = 0.69 nM 9.16
CHEMBL2058756 IC50 = 0.72 nM 9.14
CHEMBL2058757 IC50 = 0.77 nM 9.11
CHEMBL2058974 IC50 = 0.79 nM 9.10
CHEMBL2058973 IC50 = 0.83 nM 9.08
CHEMBL2058969 IC50 = 1.0 nM 9.00
CHEMBL2058761 IC50 = 1.03 nM 8.99
CHEMBL2058760 IC50 = 1.1 nM 8.96
CHEMBL399610 IC50 = 1.1 nM 8.96
CHEMBL2058759 IC50 = 1.3 nM 8.89
CHEMBL2058970 IC50 = 1.5 nM 8.82
CHEMBL2058967 IC50 = 1.5 nM 8.82
CHEMBL2058968 IC50 = 3.0 nM 8.52