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Assay Detail

CHEMBL2060155

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of BuChE in Wistar rat plasma using acetylthiocholine iodide as substrate after 0.5 hrs by Ellman's method
32
Total Activities
16
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Plasma
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Butyrylcholinesterase (CHEMBL3403)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Synthesis of phenserine analogues and evaluation of their cholinesterase inhibitory activities.
Shinada M, Narumi F, Osada Y, Matsumoto K, Yoshida T, Higuchi K, Kawasaki T, Tanaka H, Satoh M.
Bioorg Med Chem (2012) 20 : 4901 -4914
PubMed 22831800 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 4.76 7.23
Inhibition 16 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL94 PHYSOSTIGMINE 3.0 2 7.23
CHEMBL2058983 2 4.52
CHEMBL54727 2 4.52
CHEMBL2059199 2 4.44
CHEMBL2058987 2 4.38
CHEMBL2058985 2 4.18
CHEMBL2058980 2 4.06
CHEMBL2058976 2 -
CHEMBL2058978 2 -
CHEMBL2058977 2 -
CHEMBL2058979 2 -
CHEMBL2058981 2 -
CHEMBL2058982 2 -
CHEMBL2058984 2 -
CHEMBL2058986 2 -
CHEMBL2059200 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL94 PHYSOSTIGMINE IC50 = 58.4 nM 7.23
CHEMBL2058983 IC50 = 30000.0 nM 4.52
CHEMBL54727 IC50 = 30000.0 nM 4.52
CHEMBL2059199 IC50 = 36200.0 nM 4.44
CHEMBL2058987 IC50 = 41900.0 nM 4.38
CHEMBL2058985 IC50 = 65300.0 nM 4.18
CHEMBL2058980 IC50 = 87300.0 nM 4.06
CHEMBL2059199 Inhibition - % -
CHEMBL2058978 IC50 > 10000.0 nM -
CHEMBL2059200 Inhibition - % -
CHEMBL54727 Inhibition - % -
CHEMBL2058976 IC50 > 100000.0 nM -
CHEMBL2059200 IC50 > 100000.0 nM -
CHEMBL2058986 IC50 > 100000.0 nM -
CHEMBL2058984 IC50 > 100000.0 nM -
CHEMBL2058982 IC50 > 30000.0 nM -
CHEMBL2058981 IC50 > 100000.0 nM -
CHEMBL2058979 IC50 > 100000.0 nM -
CHEMBL2058977 IC50 > 10000.0 nM -
CHEMBL2058976 Inhibition - % -
CHEMBL2058978 Inhibition - % -
CHEMBL2058977 Inhibition - % -
CHEMBL2058979 Inhibition - % -
CHEMBL2058981 Inhibition - % -
CHEMBL2058980 Inhibition - % -
CHEMBL2058982 Inhibition - % -
CHEMBL2058983 Inhibition - % -
CHEMBL2058984 Inhibition - % -
CHEMBL2058985 Inhibition - % -
CHEMBL2058986 Inhibition - % -
CHEMBL94 PHYSOSTIGMINE Inhibition - % -
CHEMBL2058987 Inhibition - % -