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Assay Detail

CHEMBL2060286

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]GR113808 from human recombinant 5HT4C receptor expressed in HEK293 cells
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

5-hydroxytryptamine receptor 4 (CHEMBL1875)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery, oral pharmacokinetics and in vivo efficacy of a highly selective 5-HT4 receptor agonist: clinical compound TD-2749.
Long DD, Armstrong SR, Beattie DT, Choi SK, Fatheree PR, Gendron RA, Goldblum AA, Humphrey PP, Marquess DG, Shaw JP, Smith JA, Derek Turner S, Vickery RG.
Bioorg Med Chem Lett (2012) 22 : 4849 -4853
PubMed 22683222 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 8.34 9.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2059582 1 9.10
CHEMBL2059578 1 9.00
CHEMBL2059580 1 9.00
CHEMBL2059584 1 8.80
CHEMBL2059577 1 8.70
CHEMBL2059588 1 8.60
CHEMBL2059586 1 8.60
CHEMBL2059583 1 8.60
CHEMBL2059585 1 8.40
CHEMBL2059579 1 8.40
CHEMBL2059581 1 8.40
CHEMBL76370 TEGASEROD 4.0 1 8.40
CHEMBL2059587 1 8.10
CHEMBL2059590 1 8.00
CHEMBL538635 1 7.90
CHEMBL2059589 1 7.90
CHEMBL550064 1 7.20
CHEMBL74656 REL-CISAPRIDE 1 7.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2059582 Ki = 0.7943 nM 9.10
CHEMBL2059578 Ki = 1.0 nM 9.00
CHEMBL2059580 Ki = 1.0 nM 9.00
CHEMBL2059584 Ki = 1.585 nM 8.80
CHEMBL2059577 Ki = 1.995 nM 8.70
CHEMBL2059588 Ki = 2.512 nM 8.60
CHEMBL2059586 Ki = 2.512 nM 8.60
CHEMBL2059583 Ki = 2.512 nM 8.60
CHEMBL2059585 Ki = 3.981 nM 8.40
CHEMBL2059579 Ki = 3.981 nM 8.40
CHEMBL2059581 Ki = 3.981 nM 8.40
CHEMBL76370 TEGASEROD Ki = 3.981 nM 8.40
CHEMBL2059587 Ki = 7.943 nM 8.10
CHEMBL2059590 Ki = 10.0 nM 8.00
CHEMBL538635 Ki = 12.59 nM 7.90
CHEMBL2059589 Ki = 12.59 nM 7.90
CHEMBL550064 Ki = 63.1 nM 7.20
CHEMBL74656 REL-CISAPRIDE Ki = 100.0 nM 7.00