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Assay Detail

CHEMBL2066010

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant AChE assessed as dissociation constant for enzyme-inhibitor-substrate complex using acetylthiocholine substrate preincubated for 5 mins before substrate addition by Ellman's method
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL220)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis, design and biological evaluation of novel highly potent tacrine congeners for the treatment of Alzheimer's disease.
Hamulakova S, Janovec L, Hrabinova M, Kristian P, Kuca K, Banasova M, Imrich J.
Eur J Med Chem (2012) 55 : 23 -31
PubMed 22818849 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 9 7.41 9.50

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2064464 1 9.50
CHEMBL2064465 1 8.43
CHEMBL2064470 1 7.61
CHEMBL2064468 1 7.32
CHEMBL2064404 1 7.12
CHEMBL2064466 1 7.07
CHEMBL1677 TACRINE HYDROCHLORIDE 4.0 1 7.00
CHEMBL1256415 1 5.20
CHEMBL2064467 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2064464 Ki = 0.318 nM 9.50
CHEMBL2064465 Ki = 3.73 nM 8.43
CHEMBL2064470 Ki = 24.7 nM 7.61
CHEMBL2064468 Ki = 47.6 nM 7.32
CHEMBL2064404 Ki = 76.1 nM 7.12
CHEMBL2064466 Ki = 85.5 nM 7.07
CHEMBL1677 TACRINE HYDROCHLORIDE Ki = 101.0 nM 7.00
CHEMBL1256415 Ki = 6340.0 nM 5.20
CHEMBL2064467 Ki - -