Assay Detail
Functional
CHEMBL2066802
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Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFNgamma-stimulated NO production pretreated for 2 hrs before IFNgamma challenge measured after 24 hrs by Griess reaction
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Mus musculus |
| Cell Type | RAW264.7 |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
Synthesis, chemical reactivity as Michael acceptors, and biological potency of monocyclic cyanoenones, novel and highly potent anti-inflammatory and cytoprotective agents.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 7.10 | 8.24 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL2064456 | — | — | 1 | 8.24 |
| CHEMBL2064457 | — | — | 1 | 8.14 |
| CHEMBL1093059 | BARDOXOLONE | 2.0 | 1 | 7.78 |
| CHEMBL2064461 | — | — | 1 | 7.24 |
| CHEMBL2064462 | — | — | 1 | 6.82 |
| CHEMBL2064458 | — | — | 1 | 6.77 |
| CHEMBL2064460 | — | — | 1 | 6.66 |
| CHEMBL2064459 | — | — | 1 | 6.52 |
| CHEMBL2064463 | — | — | 1 | 5.72 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL2064456 | — | IC50 | = | 5.8 | nM | 8.24 |
| CHEMBL2064457 | — | IC50 | = | 7.3 | nM | 8.14 |
| CHEMBL1093059 | BARDOXOLONE | IC50 | = | 16.5 | nM | 7.78 |
| CHEMBL2064461 | — | IC50 | = | 58.0 | nM | 7.24 |
| CHEMBL2064462 | — | IC50 | = | 151.9 | nM | 6.82 |
| CHEMBL2064458 | — | IC50 | = | 169.9 | nM | 6.77 |
| CHEMBL2064460 | — | IC50 | = | 219.4 | nM | 6.66 |
| CHEMBL2064459 | — | IC50 | = | 304.8 | nM | 6.52 |
| CHEMBL2064463 | — | IC50 | = | 1901.4 | nM | 5.72 |