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Assay Detail

CHEMBL2072997

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant GST-tagged PI3K p110alpha using [32P]ATP after 60 mins by scintillation proximity assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Development of isoform selective PI3-kinase inhibitors as pharmacological tools for elucidating the PI3K pathway.
Bruce I, Akhlaq M, Bloomfield GC, Budd E, Cox B, Cuenoud B, Finan P, Gedeck P, Hatto J, Hayler JF, Head D, Keller T, Kirman L, Leblanc C, Le Grand D, McCarthy C, O'Connor D, Owen C, Oza MS, Pilgrim G, Press NE, Sviridenko L, Whitehead L.
Bioorg Med Chem Lett (2012) 22 : 5445 -5450
PubMed 22863202 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 18 7.01 8.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2071338 1 8.30
CHEMBL2071329 1 7.96
CHEMBL2071337 1 7.70
CHEMBL2071332 1 7.55
CHEMBL2071333 1 7.52
CHEMBL2071328 1 7.46
CHEMBL2071331 1 7.35
CHEMBL2071336 1 7.30
CHEMBL2071340 1 6.96
CHEMBL2071326 1 6.96
CHEMBL2071330 1 6.91
CHEMBL1986603 1 6.82
CHEMBL2071335 1 6.73
CHEMBL2071334 1 6.72
CHEMBL2071327 1 6.70
CHEMBL2071342 1 5.85
CHEMBL2069328 1 5.72
CHEMBL2071341 1 5.67

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2071338 Ki = 5.0 nM 8.30
CHEMBL2071329 Ki = 11.0 nM 7.96
CHEMBL2071337 Ki = 20.0 nM 7.70
CHEMBL2071332 Ki = 28.0 nM 7.55
CHEMBL2071333 Ki = 30.0 nM 7.52
CHEMBL2071328 Ki = 35.0 nM 7.46
CHEMBL2071331 Ki = 45.0 nM 7.35
CHEMBL2071336 Ki = 50.0 nM 7.30
CHEMBL2071340 Ki = 110.0 nM 6.96
CHEMBL2071326 Ki = 110.0 nM 6.96
CHEMBL2071330 Ki = 122.0 nM 6.91
CHEMBL1986603 Ki = 150.0 nM 6.82
CHEMBL2071335 Ki = 187.0 nM 6.73
CHEMBL2071334 Ki = 190.0 nM 6.72
CHEMBL2071327 Ki = 200.0 nM 6.70
CHEMBL2071342 Ki = 1400.0 nM 5.85
CHEMBL2069328 Ki = 1890.0 nM 5.72
CHEMBL2071341 Ki = 2130.0 nM 5.67