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Assay Detail

CHEMBL2073174

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of VDR-LBD expressed in human HEK293T cells co-expressing GAL4-DBD assessed as inhibition of 1,25-(OH)2D3-induced transcription after 24 hrs by FRET based GeneBLAzer assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK-293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Vitamin D3 receptor (CHEMBL1977)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of the first irreversible small molecule inhibitors of the interaction between the vitamin D receptor and coactivators.
Nandhikonda P, Lynt WZ, McCallum MM, Ara T, Baranowski AM, Yuan NY, Pearson D, Bikle DD, Guy RK, Arnold LA.
J Med Chem (2012) 55 : 4640 -4651
PubMed 22563729 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.25 5.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2070841 1 5.80
CHEMBL2070844 1 5.58
CHEMBL2070842 1 5.28
CHEMBL2070858 1 5.26
CHEMBL2070839 1 5.24
CHEMBL2070859 1 5.22
CHEMBL2070843 1 5.21
CHEMBL1626334 1 5.17
CHEMBL2070850 1 5.04
CHEMBL1528294 1 4.97
CHEMBL2070840 1 4.94

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2070841 IC50 = 1600.0 nM 5.80
CHEMBL2070844 IC50 = 2600.0 nM 5.58
CHEMBL2070842 IC50 = 5300.0 nM 5.28
CHEMBL2070858 IC50 = 5500.0 nM 5.26
CHEMBL2070839 IC50 = 5700.0 nM 5.24
CHEMBL2070859 IC50 = 6000.0 nM 5.22
CHEMBL2070843 IC50 = 6200.0 nM 5.21
CHEMBL1626334 IC50 = 6800.0 nM 5.17
CHEMBL2070850 IC50 = 9100.0 nM 5.04
CHEMBL1528294 IC50 = 10600.0 nM 4.97
CHEMBL2070840 IC50 = 11500.0 nM 4.94