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Assay Detail

CHEMBL2076045

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
TP_TRANSPORTER: inhibition of Digoxin transepithelial transport (basal to apical) (Digoxin: 0.1 uM) in MDR1-expressing LLC-PK1 cells
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Cell Type LLC-PK1
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Effects of 12 Ca2+ antagonists on multidrug resistance, MDR1-mediated transport and MDR1 mRNA expression.
Takara K, Sakaeda T, Tanigawara Y, Nishiguchi K, Ohmoto N, Horinouchi M, Komada F, Ohnishi N, Yokoyama T, Okumura K.
Eur J Pharm Sci (2002) 16 : 159 -165
PubMed 12128170 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 4.79 5.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1484 NICARDIPINE 4.0 1 5.34
CHEMBL1085699 MANIDIPINE 6300 3.0 1 5.33
CHEMBL2218858 1 5.30
CHEMBL2103761 BARNIDIPINE 2.0 1 4.90
CHEMBL2074922 EFONIDIPINE 2.0 1 4.89
CHEMBL517427 NILVADIPINE 3.0 1 4.75
CHEMBL1726 NISOLDIPINE 4.0 1 4.36
CHEMBL475534 NITRENDIPINE 3.0 1 4.17
CHEMBL23 DILTIAZEM 4.0 1 4.11
CHEMBL193 NIFEDIPINE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1484 NICARDIPINE IC50 = 4540.0 nM 5.34
CHEMBL1085699 MANIDIPINE 6300 IC50 = 4650.0 nM 5.33
CHEMBL2218858 IC50 = 4960.0 nM 5.30
CHEMBL2103761 BARNIDIPINE IC50 = 12600.0 nM 4.90
CHEMBL2074922 EFONIDIPINE IC50 = 13000.0 nM 4.89
CHEMBL517427 NILVADIPINE IC50 = 18000.0 nM 4.75
CHEMBL1726 NISOLDIPINE IC50 = 44100.0 nM 4.36
CHEMBL475534 NITRENDIPINE IC50 = 68200.0 nM 4.17
CHEMBL23 DILTIAZEM IC50 = 77700.0 nM 4.11
CHEMBL193 NIFEDIPINE IC50 = 472000.0 nM -