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Assay Detail

CHEMBL2089414

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human CRF1 receptor expressed in HEK293 cells assessed as inhibition of CRF-stimulated cAMP accumulation after 30 mins by fluorescence analysis
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Corticotropin-releasing factor receptor 1 (CHEMBL1800)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of pyrazolo[1,5-a]pyridine derivatives: potent and orally active antagonists of corticotropin-releasing factor 1 receptor.
Takahashi Y, Hibi S, Hoshino Y, Kikuchi K, Shin K, Murata-Tai K, Fujisawa M, Ino M, Shibata H, Yonaga M.
J Med Chem (2012) 55 : 5255 -5269
PubMed 22587443 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.08 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2087562 1 7.70
CHEMBL2087567 1 7.64
CHEMBL2087574 1 7.52
CHEMBL2087796 1 7.41
CHEMBL2087575 1 7.30
CHEMBL2087794 1 7.28
CHEMBL2087553 1 7.23
CHEMBL2087554 1 7.05
CHEMBL2087550 1 7.00
CHEMBL2087552 1 7.00
CHEMBL2087573 1 6.96
CHEMBL2087801 1 6.91
CHEMBL2087795 1 6.85
CHEMBL2087551 1 6.82
CHEMBL2087799 1 6.75
CHEMBL2087555 1 6.70
CHEMBL2087556 1 6.32

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2087562 IC50 = 20.0 nM 7.70
CHEMBL2087567 IC50 = 23.0 nM 7.64
CHEMBL2087574 IC50 = 30.0 nM 7.52
CHEMBL2087796 IC50 = 39.0 nM 7.41
CHEMBL2087575 IC50 = 50.0 nM 7.30
CHEMBL2087794 IC50 = 52.0 nM 7.28
CHEMBL2087553 IC50 = 59.0 nM 7.23
CHEMBL2087554 IC50 = 90.0 nM 7.05
CHEMBL2087550 IC50 = 100.0 nM 7.00
CHEMBL2087552 IC50 = 100.0 nM 7.00
CHEMBL2087573 IC50 = 110.0 nM 6.96
CHEMBL2087801 IC50 = 124.0 nM 6.91
CHEMBL2087795 IC50 = 140.0 nM 6.85
CHEMBL2087551 IC50 = 150.0 nM 6.82
CHEMBL2087799 IC50 = 180.0 nM 6.75
CHEMBL2087555 IC50 = 200.0 nM 6.70
CHEMBL2087556 IC50 = 480.0 nM 6.32