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Assay Detail

CHEMBL2090927

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Functional
Agonist activity at human alpha2B adrenoceptor expressed in CHO cells assessed as extracellular acidification measured after 4 mins by cytosensor microphysiometric analysis
6
Total Activities
6
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Alpha-2B adrenergic receptor (CHEMBL1942)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Low Doses of Allyphenyline and Cyclomethyline, Effective against Morphine Dependence, Elicit an Antidepressant-like Effect.
Del Bello F, Diamanti E, Giannella M, Mammoli V, Marchioro C, Mattioli L, Titomanlio F, Piergentili A, Quaglia W, Benedetti G, Varrone M, Pigini M.
ACS Med Chem Lett (2012) 3 : 535 -539
PubMed 24900506 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 5.67 6.00
pEC50 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1276140 1 6.00
CHEMBL2089156 1 5.80
CHEMBL1956195 CYCLOMETHYLINE 1 5.48
CHEMBL2089155 1 5.40
CHEMBL495095 ALLYPHENYLINE 1 -
CHEMBL1276139 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1276140 EC50 = 1000.0 nM 6.00
CHEMBL2089156 EC50 = 1584.89 nM 5.80
CHEMBL1956195 CYCLOMETHYLINE EC50 = 3311.31 nM 5.48
CHEMBL2089155 EC50 = 3981.07 nM 5.40
CHEMBL495095 ALLYPHENYLINE pEC50 - -
CHEMBL1276139 pEC50 - -